Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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Development and application of a CRISPR/Cas9 system for Bacillus licheniformis genome editing.
Cuixia Zhou,Huan Liu,Feiyan Yuan,Haonan Chai,Haikuan Wang,Fufeng Liu,Yu Li,Huitu Zhang,Fuping Lu +8 more
TL;DR: The results illustrated that the establishment of a CRISPR/Cas9 system for Bacillus licheniformis is of significance, and confirmed the system's high efficiency, and offers promise for applications in genetic modification of other industrial relevant Bacillus species.
Journal ArticleDOI
Large-Scale Transgenic Drosophila Resource Collections for Loss- and Gain-of-Function Studies.
Jonathan Zirin,Yanhui Hu,Lu-Ping Liu,Donghui Yang-Zhou,Ryan Colbeth,Dong Yan,Benjamin Scott Ewen-Campen,Rong Tao,Eric Vogt,Sara VanNest,Cooper Cavers,Christians Villalta,Aram Comjean,Jin Sun,Xia Wang,Yu Jia,Ruibao Zhu,Ping Peng,Jinchao Yu,Da Shen,Yuhao Qiu,Limmond Ayisi,Henna Ragoowansi,Ethan Fenton,Senait Efrem,Annette L. Parks,Kuniaki Saito,Shu Kondo,Liz Perkins,Stephanie E. Mohr,Jian-Quan Ni,Norbert Perrimon,Norbert Perrimon +32 more
TL;DR: The Transgenic RNAi Project (TRiP), a Drosophila melanogaster functional genomics platform at Harvard Medical School, was initiated in 2008 to generate and distribute a genome-scale collection of RNA interference (RNAi) fly stocks, and this work presents an update on these libraries of publicly available RNAi and CRISPR fly stocks.
Posted ContentDOI
Genetic screening for single-cell variability modulators driving therapy resistance
Eduardo A. Torre,Eri Arai,Sareh Bayatpour,Lauren E. Beck,Benjamin L. Emert,Sydney M. Shaffer,Ian A. Mellis,Mitchell Fane,Gretchen M. Alicea,Krista A. Budinich,Ashani T. Weeraratna,Junwei Shi,Arjun Raj +12 more
TL;DR: This work used CRISPR/Cas9 genetic screens to reveal genes that affect cell fate decisions by altering cellular plasticity across a range of functional categories and discovered a novel mode of altering resistance that, contrary to known mechanisms, pushes cells towards a more differentiated state.
Journal ArticleDOI
CRISPR Tools for Systematic Studies of RNA Regulation
Jesse M. Engreitz,Omar O. Abudayyeh,Omar O. Abudayyeh,Jonathan S. Gootenberg,Jonathan S. Gootenberg,Feng Zhang +5 more
TL;DR: How clustered regularly interspaced short palindromic repeat (CRISPR)-based technologies for directed perturbations of DNA and RNA are revealing new insights into RNA regulation is discussed.
Journal ArticleDOI
Sphingosine kinase 1: A novel independent prognosis biomarker in hepatocellular carcinoma.
TL;DR: Evidence is provided that Sphk1 promotes HCC cell proliferation and is involved in tumor progression and the data presented suggest that SphK1 may be a potential independent prognosis biomarker for the treatment of HCC.
References
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Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.