Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
Citations
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CRISPR-mediated activation of endogenous BST-2/tetherin expression inhibits wild-type HIV-1 production.
Yanzhao Zhang,Seiya Ozono,Seiya Ozono,Weitong Yao,Weitong Yao,Minoru Tobiume,Shoji Yamaoka,Satoshi Kishigami,Hideaki Fujita,Kenzo Tokunaga +9 more
TL;DR: The effect of the CRISPR-mediated pinpoint activation of endogenous expression of BST-2, a virus restriction factor with a broad antiviral spectrum, is shown, confirming that the physiological stoichiometry between host restriction factors and viral antagonists may determine the outcome of the battle with viruses.
Journal ArticleDOI
What rheumatologists need to know about CRISPR/Cas9
Gary Gibson,Maozhou Yang +1 more
TL;DR: The application of CRISPR/Cas9 for creating gene mutations — the application that initiated the current avalanche of interest — and new developments that have largely answered initial concerns about its specificity and ability to introduce new gene sequences are discussed.
Journal ArticleDOI
Long Non-coding RNAs as Local Regulators of Pancreatic Islet Transcription Factor Genes
TL;DR: It is proposed that cis-regulatory lncRNAs could represent a molecular target for modulation of diabetes-relevant genes and discuss challenges involved in using genetic perturbations to define their function.
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Genome editing in pluripotent stem cells: research and therapeutic applications.
TL;DR: An overview of the available genome editing systems is provided and opportunities and perspectives for their application in basic research and clinical practice are discussed, with a particular focus on hPSC based research and gene therapy approaches.
Journal ArticleDOI
Prediction and diversity of tracrRNAs from type II CRISPR-Cas systems.
Te-yuan Chyou,Chris M. Brown +1 more
TL;DR: A new bioinformatic tool to predict tracrRNAs is developed that achieved a high sensitivity and specificity, and a low false discovery rate (FDR) on genome analysis, and will assist in the design of further engineered CRISPR-Cas9 systems.
References
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TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
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RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Bo Li,Colin N. Dewey +1 more
TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.