Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
About:
The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
Citations
More filters
Journal ArticleDOI
IL10RA modulates crizotinib sensitivity in NPM1-ALK+ anaplastic large cell lymphoma.
Nina Prokoph,Nicola A. Probst,Liam C. Lee,Jack M. Monahan,Jamie D. Matthews,Huan-Chang Liang,Klaas Bahnsen,Ivonne A. Montes-Mojarro,Elif Karaca-Atabay,Elif Karaca-Atabay,Geeta G. Sharma,Vikas Malik,Hugo Larose,Sorcha Forde,Stephen P. Ducray,Cosimo Lobello,Qi Wang,Shi-Lu Luan,Šárka Pospíšilová,Carlo Gambacorti-Passerini,G. A. Amos Burke,Shahid Pervez,Andishe Attarbaschi,Andrea Janíková,Hélène Pacquement,Judith Landman-Parker,Anne Lambilliotte,Gudrun Schleiermacher,Wolfram Klapper,Ralf Jauch,Wilhelm Woessmann,Gilles Vassal,Lukas Kenner,Olaf Merkel,Luca Mologni,Roberto Chiarle,Roberto Chiarle,Laurence Brugières,Birgit Geoerger,Birgit Geoerger,Isaia Barbieri,Suzanne D. Turner,Suzanne D. Turner +42 more
TL;DR: It is shown that resistance to ALK inhibition by crizotinib in ALCL can be driven by aberrant upregulation of IL10RA, which rewires the STAT3 signaling pathway bypassing otherwise critical phosphorylation by NPM1-ALK.
Journal ArticleDOI
CRISPR-based tools for plant genome engineering.
TL;DR: A review of toolkits derived from bacterial CRISPR systems for targeted mutagenesis, gene delivery and modulation of gene expression in plants, focusing on their composition and the strategies employed to reprogramme them for the recognition of specific genomic targets is presented in this paper.
Journal ArticleDOI
WP1130 reveals USP24 as a novel target in T-cell acute lymphoblastic leukemia.
Hao Luo,Bo Jing,Yu Xia,Yugen Zhang,Meng Hu,Haiyan Cai,Yin Tong,Li Zhou,Li Yang,Junmei Yang,Hu Lei,Hanzhang Xu,Chuan-Xu Liu,Ying-Li Wu +13 more
TL;DR: It is demonstrated that knockdown of USP24 but not USP9X could significantly induce growth inhibition and apoptosis of T-ALL cells and WP1130 is a promising lead compound for developing anti-T-ALL drugs.
Journal ArticleDOI
Definition and identification of small RNA sponges: Focus on miRNA sequestration
TL;DR: From this basic science, tailored oligonucleotides are detailed, developed to challenge the binding of small RNA and efficiently restore small RNA activity by preventing their sequestration on RNA-sponges.
Journal ArticleDOI
Synthetic biology: applying biological circuits beyond novel therapies
TL;DR: This review highlights some tools that were developed in which circuits were combined such that the detection of a particular event automatically triggered a specific output and highlights how some clinical tools can be very useful in basic science.
References
More filters
Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal ArticleDOI
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Bo Li,Colin N. Dewey +1 more
TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.
Journal ArticleDOI
Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.