Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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Cas-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cas9
TL;DR: An online tool, Cas-Database, a genome-wide gRNA library design tool for Cas9 nucleases from Streptococcus pyogenes (SpCas9) with an easy-to-use web interface, which provides a powerful way to select multiple optimal target sequences from thousands of genes at once for the creation of a genomes-wide library.
Journal ArticleDOI
CRISPR-based Activation of Endogenous Neurotrophic Genes in Adipose Stem Cell Sheets to Stimulate Peripheral Nerve Regeneration.
Mu Nung Hsu,Han Tsung Liao,Vu Anh Truong,Kai Lun Huang,Fu Jen Yu,Hwei Hsien Chen,Thi Kieu Nuong Nguyen,Pavel I. Makarevich,Yelena V. Parfyonova,Yu-Chen Hu +9 more
TL;DR: The potentials of the hybrid BV-delivered CRISPRa system for multiplexed activation of endogenous neurotrophic factor genes in ASC sheets to promote peripheral nerve regeneration are implicated.
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The long non-coding RNA LUCAT1 is a negative feedback regulator of interferon responses in humans.
Shiuli Agarwal,Tim Vierbuchen,Sreya Ghosh,Jennie Chan,Zhaozhao Jiang,Richard Kumaran Kandasamy,Emiliano P. Ricci,Katherine A. Fitzgerald +7 more
TL;DR: The role of the lncRNA LUCAT1 is identified which is upregulated in human myeloid cells stimulated with lipopolysaccharide and other innate immune stimuli and functions to restrain the immune response in human cells.
Journal ArticleDOI
STING-Mediated IFI16 Degradation Negatively Controls Type I Interferon Production.
Dapei Li,Rongsheng Wu,Wen Guo,Lifen Xie,Zigang Qiao,Shengchuan Chen,Shengchuan Chen,Jingfei Zhu,Chaohao Huang,Chaohao Huang,Jian Huang,Bicheng Chen,Yanghua Qin,Feng Xu,Feng Ma +14 more
TL;DR: STING-mediated negative feedback regulation of IFI16 restricts IFN-I overproduction during antiviral immunity to avoid autoimmune diseases.
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Synergistic lethality between BRCA1 and H3K9me2 loss reflects satellite derepression
Jan Padeken,Peter Zeller,Benjamin D. Towbin,Iskra Katic,Véronique Kalck,Stephen P. Methot,Susan M. Gasser,Susan M. Gasser +7 more
TL;DR: A genome-wide synthetic lethality screen shows that the BRCA1/BARD1 complex is necessary for germline viability in worms lacking MET-2 but not SET-25, and the synthetic sterility upon BRC a/Bard1 and H3K9me2 loss is directly linked to the DNA damage provoked by unscheduled satellite repeat transcription.
References
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.