Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
About:
The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
Citations
More filters
Journal ArticleDOI
Functional annotation of native enhancers with a Cas9–histone demethylase fusion
Nicola A. Kearns,Hannah Pham,Barbara Tabak,Ryan M.J. Genga,Noah J. Silverstein,Manuel Garber,René Maehr +6 more
TL;DR: A nuclease-deficient Cas9 (dCas9)–histone demethylase fusion is used to functionally characterize previously described and new enhancer elements for their roles in the embryonic stem cell state and distinguishes the mechanism of action of dCas9-LSD1 at enhancers from previous d Cas9-effectors.
Journal ArticleDOI
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation
Levi G. Lowder,Dengwei Zhang,Nicholas J. Baltes,Joseph W. Paul,Xu Tang,Xuelian Zheng,Daniel F. Voytas,Tzung-Fu Hsieh,Yong Zhang,Yiping Qi +9 more
TL;DR: A comprehensive molecular toolbox for multifaceted CRISPR/Cas9 applications in plants is developed and implemented, with a full suite of capabilities, including multiplexed gene editing and transcriptional activation or repression of plant endogenous genes.
Journal ArticleDOI
A multifunctional AAV-CRISPR-Cas9 and its host response
Wei Leong Chew,Mohammadsharif Tabebordbar,Jason Cheng,Prashant Mali,Elizabeth Y Wu,Alex Hay-Man Ng,Kexian Zhu,Amy J. Wagers,George M. Church,George M. Church +9 more
TL;DR: The results reveal that AAV–CRISPR–Cas9 evokes host responses with distinct cellular and molecular signatures, but unlike alternative delivery methods, does not induce extensive cellular damage in vivo.
Journal ArticleDOI
Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting
Andrew J. Aguirre,Robin M. Meyers,Barbara A. Weir,Francisca Vazquez,Cheng-Zhong Zhang,Uri Ben-David,April Cook,Gavin Ha,William F. Harrington,Mihir B. Doshi,Maria Kost-Alimova,Stanley Gill,Han Xu,Levi D. Ali,Guozhi Jiang,Sasha Pantel,Yenarae Lee,Amy Goodale,Andrew D. Cherniack,Coyin Oh,Gregory V. Kryukov,Glenn S. Cowley,Levi A. Garraway,Kimberly Stegmaier,Charles W. M. Roberts,Todd R. Golub,Matthew Meyerson,David E. Root,Aviad Tsherniak,William C. Hahn +29 more
TL;DR: It is found that the number of CRISPR/Cas9-induced DNA breaks dictates a gene-independent antiproliferative response in cells, which has practical implications for using CRISpr/cas9 to interrogate cancer gene function and illustrate that cancer cells are highly sensitive to site-specific DNA damage, which may provide a path to novel therapeutic strategies.
Journal ArticleDOI
Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
Kendall R Sanson,Ruth E Hanna,Mudra Hegde,Katherine F Donovan,Christine Strand,Meagan E Sullender,Emma W Vaimberg,Amy Goodale,David E. Root,Federica Piccioni,John G. Doench +10 more
TL;DR: The recently-described CRISPRko library (Brunello) is more effective than previously published libraries at distinguishing essential and non-essential genes, providing approximately the same perturbation-level performance improvement over GeC KO libraries as GeCKO provided over RNAi.
References
More filters
Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal ArticleDOI
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Bo Li,Colin N. Dewey +1 more
TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.
Journal ArticleDOI
Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.