Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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Long Noncoding RNA in Hematopoiesis and Immunity
TL;DR: This work discusses recent advances, future prospects, and challenges in understanding the roles of lncRNAs in immunity and immune-mediated diseases.
Journal ArticleDOI
Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing.
TL;DR: A biodegradable two-dimensional delivery platform based on loading black phosphorus nanosheets with Cas9 ribonucleoprotein engineered with three nuclear localization signals at C terminus that provides efficient genome editing and gene silencing in vitro and in-vivo at a relatively low dose as compared with other nanoparticle-based delivery platforms.
Journal ArticleDOI
CRISPR–Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity
Nicholas J. Kramer,Michael S. Haney,David W. Morgens,Ana Jovičić,Ana Jovičić,Julien Couthouis,Amy Y. Li,James Ousey,Rosanna K. Ma,Gregor Bieri,C. Kimberly Tsui,Yingxiao Shi,Nicholas T. Hertz,Marc Tessier-Lavigne,Justin K. Ichida,Michael C. Bassik,Aaron D. Gitler +16 more
TL;DR: A genome-wide CRISPR screen for suppressors and enhancers of C9ORF72 dipeptide-repeat protein toxicity identifies candidate genes involved in nucleocytoplasmic transport and other pathways including RNA processing and chromatin modification and demonstrates the promise ofCRISPR–Cas9 screens in defining mechanisms of neurodegenerative diseases.
Journal ArticleDOI
CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming
Yanxia Liu,Chen Yu,Timothy Daley,Fangyuan Wang,Fangyuan Wang,William S. Cao,Salil S. Bhate,Xueqiu Lin,Christopher J. Still,Honglei Liu,Dehua Zhao,Haifeng Wang,Xinmin Xie,Sheng Ding,Wing Hung Wong,Marius Wernig,Lei S. Qi +16 more
TL;DR: This study activated expression of all endogenous transcription factors and other regulators via a pooled CRISPRa screen in embryonic stem cells, revealing genes including epigenetic regulators such as Ezh2 that can induce neuronal fate.
Journal ArticleDOI
Epitranscriptomic Enhancement of Influenza A Virus Gene Expression and Replication
David G. Courtney,Edward M. Kennedy,Rebekah E. Dumm,Hal P. Bogerd,Kevin Tsai,Nicholas S. Heaton,Bryan R. Cullen +6 more
TL;DR: It is demonstrated that global inhibition of m6A addition inhibits IAV gene expression and replication, and overexpression of the cellular m 6A "reader" protein YTHDF2 increases IAV Gene Expression and replication and reduced IAV pathogenicity in mice.
References
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.