Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
About:
The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
Citations
More filters
Journal ArticleDOI
Folate cycle enzyme MTHFD1L confers metabolic advantages in hepatocellular carcinoma
Derek Lee,Iris Ming-Jing Xu,David Kung-Chun Chiu,Robin Kit-Ho Lai,Aki Pui-Wah Tse,Lynna Lan Li,Cheuk-Ting Law,Felice Ho-Ching Tsang,Larry Lai Wei,Cerise Yuen-Ki Chan,Chun-Ming Wong,Irene Oi-Lin Ng,Carmen Chak-Lui Wong +12 more
TL;DR: This study found that an enzyme in the folate cycle, methylenetetrahydrofolate dehydrogenase 1–like (MTHFD1L), plays an essential role in support of cancer growth, and determined that MTHFD 1L is transcriptionally activated by NRF2, a master regulator of redox homeostasis.
Journal ArticleDOI
Multi-functional genome-wide CRISPR system for high throughput genotype-phenotype mapping
TL;DR: A tri-functional CRISPR system that can engineer complex synergistic interactions in a genome-wide manner is presented and represents a powerful synthetic biology tool to investigate fundamental biological questions as well as engineer complex phenotypes for biotechnological applications.
Journal ArticleDOI
Electrogenetic cellular insulin release for real-time glycemic control in type 1 diabetic mice
Krzysztof Krawczyk,Shuai Xue,Shuai Xue,Peter Buchmann,Ghislaine Charpin-El-Hamri,Pratik Saxena,Marie-Didiée Hussherr,Jiawei Shao,Jiawei Shao,Haifeng Ye,Mingqi Xie,Mingqi Xie,Martin Fussenegger,Martin Fussenegger +13 more
TL;DR: A bioelectronic interface that uses wireless-powered electrical stimulation of cells to promote the release of insulin is described, and electrosensitive human β cells are engineered to respond to membrane depolarization by rapidly releasing insulin from intracellular storage vesicles.
Journal ArticleDOI
Emerging roles of long non-coding RNAs in plant response to biotic and abiotic stresses.
Naghmeh Nejat,Nitin Mantri +1 more
TL;DR: The present knowledge of long non-coding RNAs is summarized, the recent advances in understanding regulatory functions of lncRNAs are reviewed, and the emerging roles of lNCRNAs in regulating immune responses in plants are highlighted.
Journal ArticleDOI
Current and Future Treatment Strategies for Rhabdomyosarcoma.
TL;DR: The current frontline multi-modality therapy for RMS is summarized according to pediatric protocols, emerging targeted therapies and immunotherapies identified by preclinical studies are highlighted, and early clinical trial data and the implications they hold for future clinical development are discussed.
References
More filters
Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal ArticleDOI
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Bo Li,Colin N. Dewey +1 more
TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.
Journal ArticleDOI
Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.