Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
Citations
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Journal ArticleDOI
The New State of the Art: Cas9 for Gene Activation and Repression.
Marie La Russa,Lei S. Qi +1 more
TL;DR: The recent advances of CRISPR-Cas9 technology for gene regulation are described and the advantages and disadvantages of CRisPRa and CRISpri (CRISPRa/i) relative to alternative technologies are outlined.
Applications of CRISPR–Cas systems in neuroscience
Matthias Heidenreich,Feng Zhang +1 more
TL;DR: This paper proposed a CRISPR-Cas (clustered regularly interspaced short palindromic repeat (CRISPR)-CRisPR-associated protein) system for neuroscience research.
Journal ArticleDOI
CRISPR/Cas9 advances engineering of microbial cell factories
TL;DR: This review will provide a perspective on the immediate challenges and opportunities foreseen by the use of CRISPR/Cas9 genome engineering and regulation in the context of metabolic engineering.
The long noncoding RNA Wisper controls cardiac fibrosis and remodeling
Rudi Micheletti,Isabelle Plaisance,Brian J. Abraham,Alexandre Sarre,Ching-Chia Ting,Michael Alexanian,Daniel Maric,Damien Maison,Mohamed Nemir,Blanche Schroen,Arantxa González,Samir Ounzain,Thierry Pedrazzini,Richard A. Young +13 more
TL;DR: Wisper is identified as a cardiac fibroblast–enriched super-enhancer–associated lncRNA that represents an attractive therapeutic target to reduce the pathological development of cardiac fibrosis in response to MI and prevent adverse remodeling in the damaged heart.
Journal ArticleDOI
Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis
Richard Y. Ebright,Sooncheol Lee,Ben S. Wittner,Kira L. Niederhoffer,Benjamin Nicholson,Aditya Bardia,Samuel S. Truesdell,Devon F. Wiley,Benjamin Wesley,Selena S. Li,Andy Mai,Nicola Aceto,Nicole Vincent-Jordan,Annamaria Szabolcs,Brian Chirn,Johannes Kreuzer,Valentine Comaills,Mark Kalinich,Wilhelm Haas,David T. Ting,Mehmet Toner,Mehmet Toner,Shobha Vasudevan,Daniel A. Haber,Daniel A. Haber,Shyamala Maheswaran,Douglas S. Micalizzi +26 more
TL;DR: An in vivo genome-wide CRISPR activation screen in CTCs from breast cancer patients was conducted to identify genes that promote distant metastasis in mice and found that cells with increased expression levels of certain ribosomal proteins and regulators of translation had greater metastatic capacity in a mouse model.
References
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Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal ArticleDOI
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Bo Li,Colin N. Dewey +1 more
TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.
Journal ArticleDOI
Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.