Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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The COMET toolkit for composing customizable genetic programs in mammalian cells
Patrick S. Donahue,Joseph W. Draut,Joseph J. Muldoon,Hailey I. Edelstein,Neda Bagheri,Joshua N. Leonard +5 more
TL;DR: The COmposable Mammalian Elements of Transcription (COMET) is developed, an ensemble of TFs and promoters that enable the design and tuning of gene expression to an extent not, to the best of the authors' knowledge, previously possible.
Journal ArticleDOI
CRISPR/dCas9-mediated Transcriptional Improvement of the Biosynthetic Gene Cluster for the Epothilone Production in Myxococcus Xanthus
Ran Peng,Ran Peng,Ye Wang,Wan-wan Feng,Xin-Jing Yue,Jiang-he Chen,Xiao-zhuang Hu,Zhi-feng Li,Duo-hong Sheng,Youming Zhang,Yue-zhong Li +10 more
TL;DR: The results showed that the CRISPR/dCas-mediated transcription activation is a simple and broadly applicable technique to improve the transcriptional efficiency for the production of secondary metabolites in microorganisms.
Journal ArticleDOI
Next-generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM
Yu Jia,Rong-Gang Xu,Xingjie Ren,Ben Ewen-Campen,Rajendhran Rajakumar,Jonathan Zirin,Donghui Yang-Zhou,Ruibao Zhu,Fang Wang,Decai Mao,Ping Peng,Huan-Huan Qiao,Xia Wang,Lu-Ping Liu,Lu-Ping Liu,Bowen Xu,Jun-Yuan Ji,Qingfei Liu,Jin Sun,Norbert Perrimon,Norbert Perrimon,Jian-Quan Ni +21 more
TL;DR: FlySAM greatly improves on existing in vivo CRISPRa techniques in terms of potency, scalability, and ease of use, and provides a simple and general method for conducting overexpression experiments and screens.
Journal ArticleDOI
Human Induced Pluripotent Stem-Cell-Derived Cardiomyocytes as Models for Genetic Cardiomyopathies
Andreas Brodehl,Hans Ebbinghaus,Marcus-André Deutsch,Jan Gummert,Anna Gärtner,Sandra Ratnavadivel,Hendrik Milting +6 more
TL;DR: This review provides an overview about the genetic landscape of inherited cardiomyopathies with a focus on iPSC technology, which might be of interest for clinicians and basic scientists interested in genetic cardiomeopathies.
Journal ArticleDOI
Human myotube formation is determined by MyoD-Myomixer/Myomaker axis.
TL;DR: This study uncovered the key molecular apparatus and the transcriptional control mechanism underlying human myoblast fusion, and defined the E-box motifs on promoters of Myomixer and Myomaker by which MyoD induces their expression for multinucleations of human muscle cells.
References
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The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.