Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
Citations
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RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
Pratiksha I. Thakore,Jennifer B. Kwon,Christopher E. Nelson,Douglas C. Rouse,Matthew Gemberling,Matthew L. Oliver,Charles A. Gersbach +6 more
TL;DR: AAV8 is used to deliver the transcriptional repressor dSaCas9KRAB to the cholesterol regulator Pcsk9, and repression up to 24 weeks and reduced cholesterol levels in mice are shown.
Journal ArticleDOI
Potential of Genome Editing to Improve Aquaculture Breeding and Production
TL;DR: The high fecundity and external fertilization of most aquaculture species can facilitate genome editing for research and application at a scale that is not possible in farmed terrestrial animals.
Journal ArticleDOI
Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology
TL;DR: How CRISPR can be used to build synthetic gene circuits is reviewed and recent advances inCRISPR-mediated gene regulation are discussed that offer the potential to build increasingly complex, programmable, and efficient gene circuits in the future.
Journal ArticleDOI
Rewiring human cellular input-output using modular extracellular sensors.
TL;DR: This work developed MESA receptors that enable cells to sense vascular endothelial growth factor and, in response, secrete interleukin 2, and created an immune cell that responds to a normally immunosuppressive cue by producing an immunostimulatory factor (IL-2).
Journal ArticleDOI
Multistage Delivery Nanoparticle Facilitates Efficient CRISPR/dCas9 Activation and Tumor Growth Suppression In Vivo
Qi Liu,Kai Zhao,Chun Wang,Zhanzhan Zhang,Chunxiong Zheng,Yu Zhao,Yadan Zheng,Chaoyong Liu,Yingli An,Linqi Shi,Chunsheng Kang,Yang Liu +11 more
TL;DR: The feasibility of utilizing MDNP to achieve tumor‐targeted delivery of CRISPR/dCas9 with sufficient levels to realize its therapeutic effects is suggested, suggesting the MNDP overcoming multiple physiological barriers and delivering the payload to tumor tissues with an optimal efficiency.
References
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TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.