Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
Citations
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Guide RNAs with embedded barcodes boost CRISPR-pooled screens
Shiyou Zhu,Zhongzheng Cao,Zhiheng Liu,He Yuan,Yinan Wang,Pengfei Yuan,Wei Li,Wei Li,Feng Tian,Ying Bao,Wensheng Wei +10 more
TL;DR: The iBAR approach reduces the starting cells at high MOI significantly with greatly improved efficiency and accuracy compared with the canonical CRISPR screens at a low MOI.
Journal ArticleDOI
A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence.
Bin Lu,Olaf Klingbeil,Yusuke Tarumoto,Tim D.D. Somerville,Yu-Han Huang,Yiliang Wei,Dorothy C.C. Wai,Jason Low,Joseph P. Milazzo,Xiaoli S. Wu,Zhendong Cao,Xiaomei Yan,Osama El Demerdash,Gang Huang,Joel P. Mackay,Justin B. Kinney,Junwei Shi,Christopher R. Vakoc +17 more
TL;DR: It is shown that the critical function of ZFP64 in leukemia is to maintain MLL expression via binding to the MLL promoter, which is the most enriched location of Z FP64 occupancy in the human genome.
Journal ArticleDOI
CRISPR-Cas9 technology: applications in genome engineering, development of sequence-specific antimicrobials, and future prospects
TL;DR: The origins and mechanistic basis of CRISPR-Cas systems are reviewed, how this technology can be leveraged to provide a range of applications in both eukaryotic and prokaryotic systems are discussed, and limitations and future prospects are outlined.
Journal ArticleDOI
Specific induction of endogenous viral restriction factors using CRISPR/Cas-derived transcriptional activators.
TL;DR: It is demonstrated that Cas9-derived transcriptional activators have the potential to be used for screens for endogenous genes that affect virus replication and raise the possibility that synthetic transcription factors might prove clinically useful if efficient delivery mechanisms could be developed.
Journal ArticleDOI
Gene Regulatory Network Analysis and Engineering Directs Development and Vascularization of Multilineage Human Liver Organoids
Jeremy J. Velazquez,Jeremy J. Velazquez,Ryan LeGraw,Ryan LeGraw,Farzaneh Moghadam,Farzaneh Moghadam,Yuqi Tan,Jacquelyn Kilbourne,Joseph C. Maggiore,Joshua Hislop,Silvia Liu,Davy Cats,Susana M. Chuva de Sousa Lopes,Christopher L. Plaisier,Patrick Cahan,Patrick Cahan,Samira Kiani,Samira Kiani,Mo R. Ebrahimkhani +18 more
TL;DR: The engineered tissues possess superior liver identity when compared with other PSC-derived liver organoids and show the presence of hepatocyte, biliary, endothelial, and stellate-like cell populations in single-cell RNA-seq analysis.
References
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The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.