Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
About:
The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
Citations
More filters
Journal ArticleDOI
Identification of LncRNA Linc00513 Containing Lupus-Associated Genetic Variants as a Novel Regulator of Interferon Signaling Pathway.
Zhixin Xue,Chaojie Cui,Zhuojun Liao,Shiwei Xia,Pingjing Zhang,Jialin Qin,Qiang Guo,Sheng Chen,Qiong Fu,Zhihua Yin,Zhizhong Ye,Yuanjia Tang,Nan Shen +12 more
TL;DR: The data identify two functional promoter variants of linc00513 that contribute to increased level of l inc00513 and confer susceptibility on SLE and extends the role of lncRNAs in the pathogenesis of SLE.
Journal ArticleDOI
Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors.
TL;DR: A toolkit of conditionally degradable or stabilisable orthologous dCas9 or Cpf1 effector proteins is generated, opening options for multidimensional control of functional activities through combinations of orthogonal, drug-tunable artificial transcription factors.
Journal ArticleDOI
SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds
Chao Li,Yuan Zong,Shuai Jin,Haocheng Zhu,Dexing Lin,Shengnan Li,Jin-Long Qiu,Yanpeng Wang,Caixia Gao +8 more
TL;DR: A CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminases and adenosine deaminase to Cas9 nickase target sites to generate multiplexed base editing.
Journal ArticleDOI
Modulating the expression of long non‐coding RNAs for functional studies
TL;DR: A collection of different methods used to modulate lncRNA expression levels for the assessment of biological function are surveyed, from RNA‐targeted strategies, genetic deletions, to engineered gene regulatory systems.
Journal ArticleDOI
HELLS Regulates Chromatin Remodeling and Epigenetic Silencing of Multiple Tumor Suppressor Genes in Human Hepatocellular Carcinoma.
Cheuk-Ting Law,Cheuk-Ting Law,Lai Wei,Lai Wei,Felice Ho-Ching Tsang,Felice Ho-Ching Tsang,Cerise Yuen-Ki Chan,Cerise Yuen-Ki Chan,Iris Ming-Jing Xu,Iris Ming-Jing Xu,Robin Kit-Ho Lai,Robin Kit-Ho Lai,Daniel W.H. Ho,Daniel W.H. Ho,Joyce Man-Fong Lee,Joyce Man-Fong Lee,Carmen Chak-Lui Wong,Carmen Chak-Lui Wong,Irene Oi-Lin Ng,Irene Oi-Lin Ng,Chun-Ming Wong,Chun-Ming Wong +21 more
TL;DR: The data reveal that HELLS is a key epigenetic driver of HCC; by altering the nucleosome occupancy at the NFR and enhancer, HELLS epigenetically suppresses multiple tumor suppressor genes to promote HCC progression.
References
More filters
Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal ArticleDOI
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Bo Li,Colin N. Dewey +1 more
TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.
Journal ArticleDOI
Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.