Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19.
Mingfeng Liao,Yang Liu,Jing Yuan,Yanling Wen,Gang Xu,Juanjuan Zhao,Lin Cheng,Jinxiu Li,Xin Wang,Fuxiang Wang,Lei Liu,Ido Amit,Shuye Zhang,Zheng Zhang +13 more
TLDR
Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8 + T cells in Patients with moderate CO VID-19.Abstract:
Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8+ T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.read more
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Sensing of SARS-CoV-2 by pDCs and their subsequent production of IFN-I contribute to macrophage-induced cytokine storm during COVID-19
Paôline Laurent,Chao Yang,André F. Rendeiro,Benjamin E. Nilsson-Payant,Lucia Carrau,Vasuretha Chandar,Yaron Bram,Benjamin R. tenOever,Olivier Elemento,Lionel B. Ivashkiv,Robert E. Schwartz,Franck J. Barrat +11 more
TL;DR: It is shown that the priming of macrophages can result from the response by pDCs to SARS-CoV-2, leading to macrophage activation in patients with severe COVID-19, and the uncoupling in the kinetics of the infiltration of p DCs in the lungs and the associated IFN-I signature, with the cytokine storm in Macrophages.
Journal ArticleDOI
The adaptive immune receptor repertoire community as a model for FAIR stewardship of big immunology data.
Jamie K. Scott,Felix Breden +1 more
TL;DR: High-throughput sequencing data that characterize the vast diversity of B- and T-cell clones comprising the adaptive immune receptor repertoire (AIRR-seq data) and its contribution to the understanding of COVID-19 (coronavirus disease 19) is described.
Journal ArticleDOI
Direct comparison of different therapeutic cell types susceptibility to inflammatory cytokines associated with COVID-19 acute lung injury
Ramana Vaka,Saad Khan,Bin Ye,Yousef Risha,Sandrine Parent,David W. Courtman,Duncan J. Stewart,Darryl R. Davis +7 more
TL;DR: In this paper , the impact of inflammatory cytokines on the viability, and paracrine profile (extracellular vesicles) of bone marrow derived mesenchymal stromal cells, heart-derived cells, and umbilical cord-derived mesenchyclic stroma cells were assessed using cell viability and nanoparticle tracking analysis.
Posted ContentDOI
Plasmacytoid dendritic cells produce type I interferon and reduce viral replication in airway epithelial cells after SARS-CoV-2 infection
Luisa Cervantes-Barragan,Abigail Vanderheiden,Royer Cj,Meredith E. Davis-Gardner,Philipp Ralfs,Philipp Ralfs,Tatiana Chirkova,Larry J. Anderson,Arash Grakoui,Mehul S. Suthar +9 more
TL;DR: In this article, plasmacytoid dendritic cells (pDCs) were identified as one of the key cells that can recognize SARS-CoV-2 infection, produce type I and III IFN and control viral replication in infected cells.
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