Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19.
Mingfeng Liao,Yang Liu,Jing Yuan,Yanling Wen,Gang Xu,Juanjuan Zhao,Lin Cheng,Jinxiu Li,Xin Wang,Fuxiang Wang,Lei Liu,Ido Amit,Shuye Zhang,Zheng Zhang +13 more
TLDR
Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8 + T cells in Patients with moderate CO VID-19.Abstract:
Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8+ T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.read more
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Abnormality in the NK-cell population is prolonged in severe COVID-19 patients.
Galam Leem,Shinhye Cheon,Ho-Young Lee,Seongjin Choi,Seongju Jeong,Eui-Soon Kim,Hye Won Jeong,Hyeongseok Jeong,Su-Hyung Park,Yeon Sook Kim,Eui-Cheol Shin +10 more
TL;DR: In this article, the phenotypic and functional status of NK cells and their changes during the course of mild and severe COVID-19 was examined, and it was found that the unconventional CD56dimCD16neg NK-cell population expanded in cryopreserved PBMCs from patients with COVID19 regardless of disease severity.
Journal ArticleDOI
Pulmonary Procoagulant and Innate Immune Responses in Critically Ill COVID-19 Patients
Esther J. Nossent,Alex R. Schuurman,Tom D Y Reijnders,Anno Saris,Ilse Jongerius,Siebe G. Blok,Heder J de Vries,JanWillem Duitman,Anton Vonk Noordegraaf,Lilian J. Meijboom,René Lutter,Leo M. A. Heunks,Harm Jan Bogaard,Tom van der Poll +13 more
TL;DR: In this article, the authors evaluated local and systemic activation of coagulation and interconnected inflammatory responses in critically ill patients with persistent acute respiratory distress syndrome (COVID-19) and compared with 8 healthy controls.
Journal ArticleDOI
The spike glycoprotein of SARS-CoV-2: A review of how mutations of spike glycoproteins have driven the emergence of variants with high transmissibility and immune escape
Pedro F.N. Souza,Felipe Pantoja Mesquita,Jackson L. Amaral,Patrícia G. C. Landim,Karollyny Roger Pereira Lima,Marília Braga Costa,Izabelle Rocha Farias,Mônica de Oliveira Belém,Yago O. Pinto,Heline Hellen Teixeira Moreira,Ilana Carneiro Lisboa Magalhães,Débora de Souza Collares Maia Castelo-Branco,Raquel de C. Montenegro,Claudia Roberta de Andrade +13 more
TL;DR: In this article , the authors discuss mutation of S protein and how these mutations have led to new variants that are usually more transmissible and can thus mitigate the immunity produced by vaccination.
Journal ArticleDOI
Mucosal immunity to severe acute respiratory syndrome coronavirus 2 infection.
TL;DR: The role of mucosal immune responses in the clearance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, as well as potential mucosal mechanisms of protection against (re-)infection was outlined in this article.
Journal ArticleDOI
Broad Severe Acute Respiratory Syndrome Coronavirus 2 Cell Tropism and Immunopathology in Lung Tissues From Fatal Coronavirus Disease 2019.
Suzane Ramos da Silva,Enguo Ju,Wen Meng,Alberto E. Paniz Mondolfi,Sanja Dacic,Anthony Green,Clare Bryce,Zachary Grimes,Mary Fowkes,Emilia Mia Sordillo,Carlos Cordon-Cardo,Haitao Guo,Shou-Jiang Gao +12 more
TL;DR: In this paper, the authors performed multicolor staining for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins and lineage markers to define viral tropism and lung pathobiology in 5 autopsy cases.
References
More filters
Journal ArticleDOI
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
Journal ArticleDOI
Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles
Aravind Subramanian,Pablo Tamayo,Vamsi K. Mootha,Sayan Mukherjee,Benjamin L. Ebert,Michael A. Gillette,Amanda G. Paulovich,Scott L. Pomeroy,Todd R. Golub,Eric S. Lander,Jill P. Mesirov +10 more
TL;DR: The Gene Set Enrichment Analysis (GSEA) method as discussed by the authors focuses on gene sets, that is, groups of genes that share common biological function, chromosomal location, or regulation.
Journal ArticleDOI
STAR: ultrafast universal RNA-seq aligner
Alexander Dobin,Carrie A. Davis,Felix Schlesinger,Jorg Drenkow,Chris Zaleski,Sonali Jha,Philippe Batut,Mark Chaisson,Thomas R. Gingeras +8 more
TL;DR: The Spliced Transcripts Alignment to a Reference (STAR) software based on a previously undescribed RNA-seq alignment algorithm that uses sequential maximum mappable seed search in uncompressed suffix arrays followed by seed clustering and stitching procedure outperforms other aligners by a factor of >50 in mapping speed.
Journal ArticleDOI
clusterProfiler: an R Package for Comparing Biological Themes Among Gene Clusters
TL;DR: An R package, clusterProfiler that automates the process of biological-term classification and the enrichment analysis of gene clusters and can be easily extended to other species and ontologies is presented.
Journal ArticleDOI
Comprehensive Integration of Single-Cell Data.
Tim Stuart,Andrew Butler,Paul J. Hoffman,Christoph Hafemeister,Efthymia Papalexi,William M. Mauck,Yuhan Hao,Marlon Stoeckius,Peter Smibert,Rahul Satija +9 more
TL;DR: A strategy to "anchor" diverse datasets together, enabling us to integrate single-cell measurements not only across scRNA-seq technologies, but also across different modalities.
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