Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19.
Mingfeng Liao,Yang Liu,Jing Yuan,Yanling Wen,Gang Xu,Juanjuan Zhao,Lin Cheng,Jinxiu Li,Xin Wang,Fuxiang Wang,Lei Liu,Ido Amit,Shuye Zhang,Zheng Zhang +13 more
TLDR
Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8 + T cells in Patients with moderate CO VID-19.Abstract:
Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8+ T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.read more
Citations
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Potential APOBEC-mediated RNA editing of the genomes of SARS-CoV-2 and other coronaviruses and its impact on their longer term evolution.
Jeremy Ratcliff,Peter Simmonds +1 more
TL;DR: In this article, the authors synthesize the current knowledge on APOBEC evolution and function and the evidence of their role in APOEC-mediated genome editing of SARS-CoV-2 and other coronaviruses, including extensive homoplasy from editing and reversion at targeted sites and the occurrence of driven amino acid sequence changes in viral proteins.
Journal ArticleDOI
A modified vaccinia Ankara vector-based vaccine protects macaques from SARS-CoV-2 infection, immune pathology, and dysfunction in the lungs.
Nanda Kishore Routhu,Narayanaiah Cheedarla,Narayanaiah Cheedarla,Sailaja Gangadhara,Sailaja Gangadhara,Venkata S. Bollimpelli,Venkata S. Bollimpelli,Arun K. Boddapati,Ayalnesh Shiferaw,Ayalnesh Shiferaw,Sheikh Abdul Rahman,Sheikh Abdul Rahman,Anusmita Sahoo,Anusmita Sahoo,Venkata Viswanadh Edara,Lilin Lai,Lilin Lai,Katharine Floyd,Katharine Floyd,Shelly Wang,Stephanie Fischinger,Caroline Atyeo,Sally Shin,Sanjeev Gumber,Shannon Kirejczyk,Joyce Cohen,Sherrie Jean,Jennifer S. Wood,Fawn Connor-Stroud,Rachelle L. Stammen,Amit A. Upadhyay,Kathryn L. Pellegrini,David C. Montefiori,Pei Yong Shi,Vineet D. Menachery,Galit Alter,Thomas H. Vanderford,Steven E. Bosinger,Steven E. Bosinger,Mehul S. Suthar,Mehul S. Suthar,Rama Rao Amara,Rama Rao Amara +42 more
TL;DR: In this article, modified vaccinia Ankara (MVA) vectors expressing membrane-anchored pre-fusion stabilized spike (mVA/S) but not secreted S1 induced strong neutralizing antibody responses against SARS-CoV-2 in mice.
Journal ArticleDOI
Curcumin as a Potential Treatment for COVID-19
TL;DR: In this paper, the effects of curcumin in the adjuvant treatment of SARS-CoV-2 infection were discussed and a substantial contribution was made by the use of Curcumin as a new adjuvant therapy for the treatment of COVID-19.
Journal ArticleDOI
Single-cell analyses of aging, inflammation and senescence.
Bora Uyar,Daniel Palmer,Axel Kowald,Hugo Murua Escobar,Israel Barrantes,Steffen Möller,Altuna Akalin,Georg Fuellen +7 more
TL;DR: It is suggested that single-cell experiments may hold the key to provide detailed insights whenever interventions (countering aging, inflammation, senescence, disease, etc.) are affecting cells depending on cell type.
Journal ArticleDOI
Distinct cellular immune profiles in the airways and blood of critically ill patients with COVID-19.
Anno Saris,Anno Saris,Tom D Y Reijnders,Esther J. Nossent,Alex R. Schuurman,Jan Verhoeff,Jan Verhoeff,Saskia D van Asten,Saskia D van Asten,H. J. Bontkes,Siebe G Blok,JanWillem Duitman,Harm Jan Bogaard,Leo M. A. Heunks,René Lutter,Tom van der Poll,Juan J. Garcia Vallejo,Juan J. Garcia Vallejo +17 more
TL;DR: The bronchoalveolar immune response in COVID-19 has a unique local profile that strongly differs from the immune profile in peripheral blood, which will require investigation of the pulmonary immune response.
References
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Journal ArticleDOI
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
Journal ArticleDOI
Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles
Aravind Subramanian,Pablo Tamayo,Vamsi K. Mootha,Sayan Mukherjee,Benjamin L. Ebert,Michael A. Gillette,Amanda G. Paulovich,Scott L. Pomeroy,Todd R. Golub,Eric S. Lander,Jill P. Mesirov +10 more
TL;DR: The Gene Set Enrichment Analysis (GSEA) method as discussed by the authors focuses on gene sets, that is, groups of genes that share common biological function, chromosomal location, or regulation.
Journal ArticleDOI
STAR: ultrafast universal RNA-seq aligner
Alexander Dobin,Carrie A. Davis,Felix Schlesinger,Jorg Drenkow,Chris Zaleski,Sonali Jha,Philippe Batut,Mark Chaisson,Thomas R. Gingeras +8 more
TL;DR: The Spliced Transcripts Alignment to a Reference (STAR) software based on a previously undescribed RNA-seq alignment algorithm that uses sequential maximum mappable seed search in uncompressed suffix arrays followed by seed clustering and stitching procedure outperforms other aligners by a factor of >50 in mapping speed.
Journal ArticleDOI
clusterProfiler: an R Package for Comparing Biological Themes Among Gene Clusters
TL;DR: An R package, clusterProfiler that automates the process of biological-term classification and the enrichment analysis of gene clusters and can be easily extended to other species and ontologies is presented.
Journal ArticleDOI
Comprehensive Integration of Single-Cell Data.
Tim Stuart,Andrew Butler,Paul J. Hoffman,Christoph Hafemeister,Efthymia Papalexi,William M. Mauck,Yuhan Hao,Marlon Stoeckius,Peter Smibert,Rahul Satija +9 more
TL;DR: A strategy to "anchor" diverse datasets together, enabling us to integrate single-cell measurements not only across scRNA-seq technologies, but also across different modalities.
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