Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19.
Mingfeng Liao,Yang Liu,Jing Yuan,Yanling Wen,Gang Xu,Juanjuan Zhao,Lin Cheng,Jinxiu Li,Xin Wang,Fuxiang Wang,Lei Liu,Ido Amit,Shuye Zhang,Zheng Zhang +13 more
TLDR
Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8 + T cells in Patients with moderate CO VID-19.Abstract:
Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8+ T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.read more
Citations
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Inferring microenvironmental regulation of gene expression from single-cell RNA sequencing data using scMLnet with an application to COVID-19.
TL;DR: Wang et al. as mentioned in this paper presented a single-cell RNA-sequencing data based multilayer network method (scMLnet) that models not only functional intercellular communications but also intracellular gene regulatory networks.
Journal ArticleDOI
A genetic link between risk for Alzheimer's disease and severe COVID-19 outcomes via the OAS1 gene.
Naciye Magusali,Andrew Graham,Thomas M. Piers,Pantila Panichnantakul,Umran Yaman,Maryam Shoai,Regina H. Reynolds,Regina H. Reynolds,Juan A. Botía,Keeley J. Brookes,Tamar Guetta-Baranes,Eftychia Bellou,Sevinc Bayram,Dimitra Sokolova,Mina Ryten,Mina Ryten,Carlo Sala Frigerio,Valentina Escott-Price,Kevin Morgan,Jennifer M. Pocock,John Hardy,Dervis A. Salih +21 more
TL;DR: In this paper, the same OAS1 locus has been recently associated with severe coronavirus disease 2019 (COVID-19) outcomes, linking risk for both diseases.
Journal ArticleDOI
Self-sustaining IL-8 loops drive a prothrombotic neutrophil phenotype in severe COVID-19.
Rainer Kaiser,Alexander Leunig,Kami Pekayvaz,Oliver Popp,Markus Joppich,Vivien Polewka,Raphael Escaig,Afra Anjum,Marie-Louise Hoffknecht,Christoph Gold,Sophia Brambs,Anouk Engel,Sven Stockhausen,Viktoria Knottenberg,Anna Titova,Mohamed Haji,Clemens Scherer,Maximilian Muenchhoff,Johannes C. Hellmuth,Kathrin Saar,Benjamin Schubert,Anne Hilgendorff,Christian Schulz,Stefan Kääb,Ralf Zimmer,Norbert Hubner,Steffen Massberg,Philipp Mertins,Leo Nicolai,Konstantin Stark +29 more
TL;DR: In this article, the authors identify a reinforcing loop of both systemic and neutrophil intrinsic IL-8 (CXCL8/IL-8) dysregulation, which initiates and perpetuates neutrophin-driven immunopathology.
Journal ArticleDOI
The Surviving Sepsis Campaign: Research Priorities for Coronavirus Disease 2019 in Critical Illness
Craig M. Coopersmith,Massimo Antonelli,Seth R. Bauer,Clifford S. Deutschman,Laura Evans,Ricard Ferrer,Judith Hellman,Sameer Jog,Jozef Kesecioglu,Niranjan Kissoon,Ignacio Martin-Loeches,Mark E. Nunnally,Hallie C. Prescott,Andrew Rhodes,Daniel Talmor,Pierre Tissieres,Daniel De Backer +16 more
TL;DR: The Surviving Sepsis Research Committee as mentioned in this paper identified 13 research priorities in the management, pathophysiology, and host response of coronavirus disease 2019 in critically ill patients, including the following: 1) Should the approach to ventilator management differ from the standard approach in patients with acute hypoxic respiratory failure? 2) Can the host response be modulated for therapeutic benefit? 3) What specific cells are directly targeted by severe acute respiratory syndrome coronavirus 2, and how do these cells respond? 4) Can early data be used to predict outcomes of coronaval
Posted ContentDOI
Macropahge expression and prognostic significance of the long pentraxin PTX3 in COVID-19
Enrico Brunetta,Marco Folci,Barbara Bottazzi,Maria De Santis,Alessandro Protti,Sarah N. Mapelli,Roberto Leone,Ilaria My,Monica Bacci,Veronica Zanon,Gianmarco Spata,Andrea Gianatti,Marina Sironi,Claudio Angelini,Cecilia Garlanda,Michele Ciccarelli,Maurizio Cecconi,Alberto Mantovani,Alberto Mantovani +18 more
TL;DR: The results reported here suggest that circulating and lung myelomonocytic cells are a major source of PTX3 and thatPTX3 plasma levels can serve as a strong prognostic indicator of short-term mortality in COVID-19.
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TL;DR: A strategy to "anchor" diverse datasets together, enabling us to integrate single-cell measurements not only across scRNA-seq technologies, but also across different modalities.
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