Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19.
Mingfeng Liao,Yang Liu,Jing Yuan,Yanling Wen,Gang Xu,Juanjuan Zhao,Lin Cheng,Jinxiu Li,Xin Wang,Fuxiang Wang,Lei Liu,Ido Amit,Shuye Zhang,Zheng Zhang +13 more
TLDR
Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8 + T cells in Patients with moderate CO VID-19.Abstract:
Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8+ T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.read more
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Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.
Hiroaki Katsura,Vishwaraj Sontake,Aleksandra Tata,Yoshihiko Kobayashi,Caitlin E. Edwards,Brook E. Heaton,Arvind Konkimalla,Takanori Asakura,Yu Mikami,Ethan J. Fritch,Patty J. Lee,Nicholas S. Heaton,Richard C. Boucher,Scott H. Randell,Ralph S. Baric,Purushothama Rao Tata +15 more
TL;DR: Human stem cell-based alveolospheres provide novel insights into COVID-19 pathogenesis and can serve as a model for understanding human respiratory diseases.
Journal ArticleDOI
SARS-CoV-2 T cell immunity: Specificity, function, durability, and role in protection.
TL;DR: In efforts to synthesize a clear understanding of SARS-CoV-2 protective immunity, antibody analysis has been paralleled by T cell studies across asymptomatic, mild and severe COVID-19.
Journal ArticleDOI
Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.
Peter A. Szabo,Pranay Dogra,Joshua I. Gray,Steven B. Wells,Thomas J. Connors,Stuart P. Weisberg,Izabela Krupska,Rei Matsumoto,Maya M.L. Poon,Emma Idzikowski,Sinead E. Morris,Chloé Pasin,Andrew J. Yates,Amy Ku,Michael Chait,Julia Davis-Porada,Xinzheng V. Guo,Jing Zhou,Matthew Steinle,Sean Mackay,Anjali Saqi,Matthew R. Baldwin,Peter A. Sims,Donna L. Farber +23 more
TL;DR: In this paper, the authors examined the relationship between immune processes in the respiratory tract and circulation through longitudinal phenotypic, transcriptomic, and cytokine profiling of paired airway and blood samples from patients with severe COVID-19 relative to heathy controls.
Journal ArticleDOI
The ORF8 protein of SARS-CoV-2 mediates immune evasion through down-regulating MHC-Ι.
Yiwen Zhang,Yingshi Chen,Yuzhuang Li,Feng Huang,Baohong Luo,Yaochang Yuan,Baijin Xia,Xiancai Ma,Tao Yang,Fei Yu,Jun Liu,Bingfeng Liu,Zheng Song,J. Chen,Shu-Mei Yan,Liyang Wu,Ting Pan,Xu Zhang,Rong Li,Wenjing Huang,Xin He,Fei Xiao,Junsong Zhang,Hui Zhang +23 more
TL;DR: Wang et al. as discussed by the authors showed that SARS-CoV-2 infection leads to major histocompability complex class Ι (MHC-Ι) down-regulation both in vitro and in vivo.
Journal ArticleDOI
Frontline Science: COVID-19 infection induces readily detectable morphologic and inflammation-related phenotypic changes in peripheral blood monocytes
Dan Zhang,Rui Guo,Lei Lei,Hongjuan Liu,Yawen Wang,Yi-Li Wang,Hongbo Qian,Tongxin Dai,Tianxiao Zhang,Yanjun Lai,Jingya Wang,Zhiqiang Liu,Tianyan Chen,Aili He,Michael O'Dwyer,Michael O'Dwyer,Jinsong Hu +16 more
TL;DR: Flow cytometric analysis of peripheral blood samples from 34 COVID‐19 patients in early 2020 identified significant morphologic and functional differences, which are more pronounced in patients requiring prolonged hospitalization and intensive care unit (ICU) admission.
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