Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19.
Mingfeng Liao,Yang Liu,Jing Yuan,Yanling Wen,Gang Xu,Juanjuan Zhao,Lin Cheng,Jinxiu Li,Xin Wang,Fuxiang Wang,Lei Liu,Ido Amit,Shuye Zhang,Zheng Zhang +13 more
TLDR
Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8 + T cells in Patients with moderate CO VID-19.Abstract:
Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8+ T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.read more
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Acute SARS-CoV-2 Infection Impairs Dendritic Cell and T Cell Responses
Runhong Zhou,Kelvin K. W. To,Yik Chun Wong,Li Liu,Biao Zhou,Xin Li,Haode Huang,Yufei Mo,Tsz Yat Luk,Thomas Tsz Kan Lau,Pauline Yeung,Wai-Ming Chan,Alan Ka Lun Wu,Kwok Cheung Lung,Owen Tak-Yin Tsang,Wai Shing Leung,Ivan Hung,Kwok-Yung Yuen,Zhiwei Chen +18 more
TL;DR: Investigating 17 acute and 24 convalescent patients found that acute SARS-CoV-2 infection resulted in broad immune cell reduction including T, NK, monocyte and dendritic cell (DC) and DCs were significantly reduced with functional impairment, and cDC:pDC ratios were increased among acute severe patients.
Journal ArticleDOI
T cell responses to SARS-CoV-2 spike cross-recognize Omicron
Roanne Keeton,Marius Belmondo Tincho,Amkele Ngomti,Richard Baguma,N. Benede,Akiko Suzuki,Khadija Khan,Sandile Cele,Mallory Bernstein,Farina Karim,Sharon Madzorera,Thandeka Moyo-Gwete,M. Mennen,S. Skelem,M. Adriaanse,Daniel Mutithu,Olukayode O. Aremu,Cari Stek,Elsa Du Bruyn,M. A. Van Der Mescht,Zelda de Beer,T. R. de Villiers,Anne Brunhilde Bodenstein,Gretha van den Berg,Adriano Mendes,Amy Strydom,Marietjie Venter,Jennifer Giandhari,Yeshnee Naidoo,Sureshnee Pillay,Houriiyah Tegally,Alba Grifoni,Daniela Weiskopf,Alessandro Sette,Robert J. Wilkinson,Tulio de Oliveira,Linda-Gail Bekker,Glenda Gray,Veronica Ueckermann,Theresa M. Rossouw,Michael T. Boswell,Jinal N. Bhiman,Penny L. Moore,Alex Sigal,Ntobeko A.B. Ntusi,Wendy A. Burgers,Catherine Riou +46 more
TL;DR: In this paper , the authors assessed the ability of T cells to react to Omicron spike protein in participants who were vaccinated with Ad26.S or BNT162b2, or unvaccinated convalescent COVID-19 patients.
Journal ArticleDOI
Immunological mechanisms of vaccine-induced protection against COVID-19 in humans.
TL;DR: Most COVID-19 vaccines have been designed to elicit immune responses, ideally neutralizing antibodies (NAbs), against the SARS-CoV-2 spike protein this article.
Journal ArticleDOI
Association of COVID-19 inflammation with activation of the C5a-C5aR1 axis.
Julien Carvelli,Olivier Demaria,Frédéric Vély,Luciana Batista,Nassima Chouaki Benmansour,Joanna Fares,Sabrina Carpentier,Marie-Laure Thibult,Ariane Morel,Romain Remark,Pascale Andre,Agnès Represa,Christelle Piperoglou,Pierre Yves Cordier,Erwan Le Dault,Christophe Guervilly,Pierre Simeone,Marc Gainnier,Yannis Morel,Mikael Ebbo,Nicolas Schleinitz,Eric Vivier +21 more
TL;DR: Results suggest that blockade of the C5a–C5aR1 axis could be used to limit the infiltration of myeloid cells in damaged organs and prevent the excessive lung inflammation and endothelialitis that are associated with acute respiratory distress syndrome in patients with COVID-19.
Journal ArticleDOI
The COVID-19 puzzle: deciphering pathophysiology and phenotypes of a new disease entity.
Marcin F. Osuchowski,Martin Sebastian Winkler,Tomasz Skirecki,Sara Cajander,Manu Shankar-Hari,Manu Shankar-Hari,Gunnar Lachmann,Guillaume Monneret,Fabienne Venet,Michael Bauer,Frank M. Brunkhorst,Sebastian Weis,Alberto García-Salido,Matthijs Kox,Jean-Marc Cavaillon,Florian Uhle,Markus A. Weigand,Stefanie B. Flohé,W. Joost Wiersinga,Raquel Almansa,Amanda de la Fuente,Ignacio Martin-Loeches,Christian Meisel,Thibaud Spinetti,Joerg C. Schefold,Catia Cilloniz,Antoni Torres,Antoni Torres,Evangelos J. Giamarellos-Bourboulis,Ricard Ferrer,Ricard Ferrer,Massimo Girardis,Andrea Cossarizza,Mihai G. Netea,Mihai G. Netea,Mihai G. Netea,Tom van der Poll,Jesus F. Bermejo-Martin,Ignacio Rubio +38 more
TL;DR: The authors reviewed the intricacies of COVID-19 pathophysiology, its various phenotypes, and the anti-SARS-CoV-2 host response at the humoral and cellular levels.
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TL;DR: A strategy to "anchor" diverse datasets together, enabling us to integrate single-cell measurements not only across scRNA-seq technologies, but also across different modalities.
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