Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19.
Mingfeng Liao,Yang Liu,Jing Yuan,Yanling Wen,Gang Xu,Juanjuan Zhao,Lin Cheng,Jinxiu Li,Xin Wang,Fuxiang Wang,Lei Liu,Ido Amit,Shuye Zhang,Zheng Zhang +13 more
TLDR
Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8 + T cells in Patients with moderate CO VID-19.Abstract:
Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8+ T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.read more
Citations
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SARS-CoV-2 drives JAK1/2-dependent local complement hyperactivation.
Bingyu Yan,Tilo Freiwald,Tilo Freiwald,Daniel Chauss,Luopin Wang,Erin E. West,Carmen Mirabelli,Charles J. Zhang,Eva-Maria Nichols,Nazish Malik,Richard Gregory,Marcus Bantscheff,Sonja Ghidelli-Disse,Martin Kolev,Tristan Frum,Jason R. Spence,Jonathan Z. Sexton,Konstantinos D. Alysandratos,Konstantinos D. Alysandratos,Darrell N. Kotton,Darrell N. Kotton,Stefania Pittaluga,Jack A. Bibby,Nathalie Niyonzima,Matthew R. Olson,Shahram Kordasti,Shahram Kordasti,Didier Portilla,Didier Portilla,Christiane E. Wobus,Arian Laurence,Michail S. Lionakis,Claudia Kemper,Claudia Kemper,Behdad Afzali,Majid Kazemian +35 more
TL;DR: In this paper, the authors found that the complement system was one of the intracellular pathways most highly induced by SARS-CoV-2 infection in lung epithelial cells.
Journal ArticleDOI
COVID-19: Complement, Coagulation, and Collateral Damage.
TL;DR: A summary of CO VID-19 is provided and complement’s role in COVID-19 acute respiratory distress syndrome and coagulopathy is reviewed, suggesting that the complement system plays a key role in this inflammatory reaction.
Journal ArticleDOI
Flattening the COVID-19 Curve With Natural Killer Cell Based Immunotherapies.
Marisa Market,Leonard Angka,Leonard Angka,Andre B Martel,Andre B Martel,Donald Bastin,Oladunni Olanubi,Oladunni Olanubi,Gayashan Tennakoon,Dominique M. Boucher,Juliana Ng,Michele Ardolino,Michele Ardolino,Rebecca C. Auer,Rebecca C. Auer +14 more
TL;DR: The current understanding of how NK cells respond in both early and late coronavirus infections, and the implication for ongoing COVID-19 clinical trials are summarized and recommendations for therapeutic strategies are outlined.
Posted ContentDOI
Immunological memory to SARS-CoV-2 assessed for greater than six months after infection
Jennifer M. Dan,Jennifer M. Dan,Jose Mateus,Yu Kato,Kathryn M. Hastie,Esther Dawen Yu,Caterina E. Faliti,Alba Grifoni,Sydney I. Ramirez,Sydney I. Ramirez,Sonya Haupt,April Frazier,Catherine Nakao,Catherine Nakao,Vamseedhar Rayaprolu,Stephen A. Rawlings,Bjoern Peters,Bjoern Peters,Florian Krammer,Viviana Simon,Erica Ollmann Saphire,Erica Ollmann Saphire,Davey M. Smith,Daniela Weiskopf,Alessandro Sette,Alessandro Sette,Shane Crotty,Shane Crotty +27 more
TL;DR: It is observed that each component of SARS-CoV-2 immune memory exhibited distinct kinetics, and Spike IgG was relatively stable over 6+ months.
Journal ArticleDOI
Chemokines and chemokine receptors during COVID-19 infection
TL;DR: In this paper, the role of chemokines and their receptors in COVID-19 pathogenesis was reviewed to better understand the disease immunopathology which may aid in developing possible therapeutic targets for the infection.
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