Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19.
Mingfeng Liao,Yang Liu,Jing Yuan,Yanling Wen,Gang Xu,Juanjuan Zhao,Lin Cheng,Jinxiu Li,Xin Wang,Fuxiang Wang,Lei Liu,Ido Amit,Shuye Zhang,Zheng Zhang +13 more
TLDR
Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8 + T cells in Patients with moderate CO VID-19.Abstract:
Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8+ T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.read more
Citations
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SARS-CoV-2 ORF8 and SARS-CoV ORF8ab: Genomic Divergence and Functional Convergence.
Sameer Mohammad,Abderrezak Bouchama,Bothina Mohammad Alharbi,Mamoon Rashid,Tanveer Saleem Khatlani,Nusaibah S. Gaber,Shuja Shafi Malik +6 more
TL;DR: There appears to be significant structural integrity and functional proximity between these proteins pointing towards their high significance, and there is further need for comprehensive genomics and structural-functional studies to lead towards definitive conclusions regarding their criticality.
Posted ContentDOI
SARS-CoV-2 sensing by RIG-I and MDA5 links epithelial infection to macrophage inflammation
Lucy Thorne,Ann-Kathrin Reuschl,Lorena Zuliani-Alvarez,Matthew Whelan,Mahdad Noursadeghi,Clare Jolly,Greg J. Towers +6 more
TL;DR: In this article, the authors found that SARS-CoV-2 replicates rapidly in lung epithelial cells despite triggering a robust innate immune response through activation of cytoplasmic RNA-ensors RIG-I and MDA5.
Journal ArticleDOI
Daily Viral Kinetics and Innate and Adaptive Immune Response Assessment in COVID-19: a Case Series.
Pauline Vetter,Christiane S Eberhardt,Christiane S Eberhardt,Benjamin Meyer,Paola Andrea Martinez Murillo,Giulia Torriani,Giulia Torriani,Fiona Pigny,Sylvain Lemeille,Samuel Cordey,Samuel Cordey,Florian Laubscher,Florian Laubscher,Diem-Lan Vu,Diem-Lan Vu,Adrien Calame,Manuel Schibler,Manuel Schibler,Frederique Jacquerioz,Geraldine Blanchard-Rohner,Claire-Anne Siegrist,Laurent Kaiser,Arnaud M. Didierlaurent,Arnaud M. Didierlaurent,Isabella Eckerle +24 more
TL;DR: All patients, even those with mild symptoms, mount an innate response sufficient for viral control and develop specific immunity as well as cellular and humoral SARS-CoV-2-specific adaptive responses, which already begin to decline a few months after the resolution of symptoms.
Journal ArticleDOI
SARS-CoV-2 receptor is co-expressed with elements of the kinin-kallikrein, renin-angiotensin and coagulation systems in alveolar cells.
Davi Sidarta-Oliveira,Carlos Poblete Jara,Adriano J. Ferruzzi,Munir S. Skaf,William H. Velander,Eliana P. Araújo,Licio A. Velloso +6 more
TL;DR: A healthy human lung cell atlas meta-analysis is assembled and it is shown that key elements of the bradykinin, angiotensin and coagulation systems are co-expressed with ACE2 in alveolar cells and associated with their differentiation dynamics, which could explain how changes in ACE2 promoted by SARS-CoV-2 cell entry result in the development of the three most severe clinical components of COVID-19.
Journal ArticleDOI
Chemokines and eicosanoids fuel the hyperinflammation within the lungs of patients with severe COVID-19.
Younes Zaid,Étienne Doré,Isabelle Dubuc,Anne Sophie Archambault,Olivier Flamand,Michel Laviolette,Nicolas Flamand,Eric Boilard,Louis Flamand +8 more
TL;DR: In this article, the authors analyzed and compared the plasma and bronchoalveolar lavage (BAL) fluids of patients with severe COVID-19 (n = 45) for the presence of cytokines and lipid mediators of inflammation (LMIs).
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