Annamalai University

About: Annamalai University is a education organization based out in Chidambaram, Tamil Nadu, India. It is known for research contribution in the topics: Lipid peroxidation & Antioxidant. The organization has 8098 authors who have published 10758 publications receiving 203872 citations.

Silibinin potentially attenuates arsenic-induced oxidative stress mediated cardiotoxicity and dyslipidemia in rats

Abstract: Cardiac dysfunction is one of the major causes of mortality and morbidity throughout the world. Chronic exposure of arsenic (As) mainly leads to cardiotoxic effect. Cardiotoxicity was induced by the sodium arsenite as the source of As (5 mg/kg BW, PO) for 4 weeks. As intoxication significantly (p < 0.05) increased the serum cardiac markers, viz. creatine kinase-MB, lactate dehydrogenase, aspartate transaminase, alanine transaminase and alkaline phosphatase, oxidative stress markers in heart, plasma total cholesterol (TC), triglycerides (TG), phospholipids (PL), free fatty acids (FFA), low density lipoprotein cholesterol, very low density lipoprotein cholesterol as well as cardiac lipid profile (TC, TG and FFA) and significantly (p < 0.05) decreased the level of serum high density lipoprotein cholesterol, cardiac PL, mitochondrial enzymes such as ICDH, SDH, MDH, α-KDH and NADH dehydrogenase, levels of enzymatic antioxidant, nonenzymatic antioxidants and membrane-bound ATPases in heart. In addition, As-intoxicated rats showed a significant (p < 0.05) up-regulation of myocardial NADPH (NOX) oxidase sub units such as NOX2 and NOX4 as well as Keap-1 and down-regulation of Nrf2 and HO-1 protein expressions. Pre-administration of silibinin (SB) (75 mg/kg BW) remarkably recovered all these altered parameters to near normalcy in As-induced cardiotoxic rat. Moreover, the light microscopic and transmission electron microscopic study further supports the protective efficacy of SB on the heart mitochondria. In conclusion, our data demonstrate that SB has a potential to extenuate the arsenic-induced cardiotoxicity and dyslipidemia in rat. .

GC-MS analysis of bio-active compounds in methanolic extract of Lactuca runcinata DC.

Abstract: Background: The presence of phytochemical constitutes has been reported from species of the Compositae (Asteraceae). Hitherto no reports exist on the phytochemical components and biological activity of Lactuca runcinata DC. Objective: The present study was designed to determine the bioactive compounds in the whole plant methanol extract of Lactuca runcinata. Materials and Methods: Phytochemical screening of the entire herb of Lactuca runcinata DC revealed the presence of some bio-active components. Gas chromatography-mass spectrometry (GC-MS) analysis of the whole plant methanol extract of Lactuca runcinata was performed on a GC-MS equipment (Thermo Scientific Co.) Thermo GC-TRACE ultra ver.: 5.0, Thermo MS DSQ II. Results: The phytochemical tests showed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, phlobatannin, reducing sugars, saponins, steroids, tannins, terpenoids, volatile oils, carbohydrates, and protein/amino acids in methanolic extract of L. runcinata . The GC-MS analysis has shown the presence of different phytochemical compounds in the methanolic extract of Lactuca runcinata . A total of 21 compounds were identified representing 84.49% of total methanolic extract composition. Conclusion: From the results, it is evident that Lactuca runcinata contains various phytocomponents and is recommended as a plant of phytopharmaceutical importance.

Distribution of rare earth elements and heavy metals in the surficial sediments of the Himalayan river system

Abstract: Rare earth elements (REEs) are considered as useful tracers of various surface geological processes in the river system. REEs and heavy metals (V, Pb, Cr, Co, Ag, Zn, Cd, Ni) were analyzed from the suspended sediments of Ganges and surficial sediments of Yamuna, Brahmaputra, Jamuna, Padma and Meghna, using VG Thermal Ionization Mass Spectrometer. Physical weathering process seems to be a major controlling factor for the distribution of REE and trace metals in the sediments of the Himalayan rivers. Weathering of the surface crustal area in the drainage basin shows significant variations due to changes in lithology and influence of tributaries. The distribution of REE shows an almost uniform pattern due to factors such as river transportation processes and high level of terrigenous mixing in the bed sediments. The results show that finer grain size and high content of clay mineral (illite) in these sediments are possible traps for the accumulation of metals. Anthropogenic activities seem to have very little influence in controlling the elemental distribution in the Himalayan rivers.