Showing papers by "Braunschweig University of Technology published in 2012"

Transition Metal Oxides for Organic Electronics: Energetics, Device Physics and Applications

Abstract: During the last few years, transition metal oxides (TMO) such as molybdenum tri-oxide (MoO3), vanadium pent-oxide (V2O5) or tungsten tri-oxide (WO3) have been extensively studied because of their exceptional electronic properties for charge injection and extraction in organic electronic devices. These unique properties have led to the performance enhancement of several types of devices and to a variety of novel applications. TMOs have been used to realize efficient and long-term stable p-type doping of wide band gap organic materials, charge-generation junctions for stacked organic light emitting diodes (OLED), sputtering buffer layers for semi-transparent devices, and organic photovoltaic (OPV) cells with improved charge extraction, enhanced power conversion efficiency and substantially improved long term stability. Energetics in general play a key role in advancing device structure and performance in organic electronics; however, the literature provides a very inconsistent picture of the electronic structure of TMOs and the resulting interpretation of their role as functional constituents in organic electronics. With this review we intend to clarify some of the existing misconceptions. An overview of TMO-based device architectures ranging from transparent OLEDs to tandem OPV cells is also given. Various TMO film deposition methods are reviewed, addressing vacuum evaporation and recent approaches for solution-based processing. The specific properties of the resulting materials and their role as functional layers in organic devices are discussed.

Towards Energy and Resource Efficient Manufacturing: A Processes and Systems Approach

Abstract: A B S T R A C T This paper aims to provide a systematic overview of the state of the art in energy and resource efficiency increasing methods and techniques in the domain of discrete part manufacturing, with attention for the effectiveness of the available options. For this purpose a structured approach, distinguishing different system scale levels, is applied: starting from a unit process focus, respectively the multi-machine, factory, multi-facility and supply chain levels are covered. Determined by the research contributions reported in literature, the de facto focus of the paper is mainly on energy related aspects of manufacturing. Significant opportunities for systematic efficiency improving measures are identified and summarized in this area. 2012 CIRP.

Condition-Dependent Transcriptome Reveals High-Level Regulatory Architecture in Bacillus subtilis

Abstract: Bacteria adapt to environmental stimuli by adjusting their transcriptomes in a complex manner, the full potential of which has yet to be established for any individual bacterial species. Here, we report the transcriptomes of Bacillus subtilis exposed to a wide range of environmental and nutritional conditions that the organism might encounter in nature. We comprehensively mapped transcription units (TUs) and grouped 2935 promoters into regulons controlled by various RNA polymerase sigma factors, accounting for ~66% of the observed variance in transcriptional activity. This global classification of promoters and detailed description of TUs revealed that a large proportion of the detected antisense RNAs arose from potentially spurious transcription initiation by alternative sigma factors and from imperfect control of transcription termination.

Fluorescence enhancement at docking sites of DNA-directed self-assembled nanoantennas.

Abstract: We introduce self-assembled nanoantennas to enhance the fluorescence intensity in a plasmonic hotspot of zeptoliter volume. The nanoantennas are prepared by attaching one or two gold nanoparticles (NPs) to DNA origami structures, which also incorporated docking sites for a single fluorescent dye next to one NP or in the gap between two NPs. We measured the dependence of the fluorescence enhancement on NP size and number and compare it to numerical simulations. A maximum of 117-fold fluorescence enhancement was obtained for a dye molecule positioned in the 23-nanometer gap between 100-nanometer gold NPs. Direct visualization of the binding and unbinding of short DNA strands, as well as the conformational dynamics of a DNA Holliday junction in the hotspot of the nanoantenna, show the compatibility with single-molecule assays.

Photophysics of Fluorescent Probes for Single-Molecule Biophysics and Super-Resolution Imaging

Abstract: Single-molecule fluorescence spectroscopy and super-resolution microscopy are important elements of the ongoing technical revolution to reveal biochemical and cellular processes in unprecedented clarity and precision. Demands placed on the photophysical properties of the fluorophores are stringent and drive the choice of appropriate probes. Such fluorophores are not simple light bulbs of a certain color and brightness but instead have their own “personalities” regarding spectroscopic parameters, redox properties, size, water solubility, photostability, and several other factors. Here, we review the photophysics of fluorescent probes, both organic fluorophores and fluorescent proteins, used in applications such as particle tracking, single-molecule FRET, stoichiometry determination, and super-resolution imaging. Of particular interest is the thiol-induced blinking of Cy5, a curse for single-molecule biophysical studies that was later overcome using Trolox through a reducing/oxidizing system but a boon for ...

GaN based nanorods for solid state lighting

Abstract: In recent years, GaN nanorods are emerging as a very promising novel route toward devices for nano-optoelectronics and nano-photonics. In particular, core-shell light emitting devices are thought to be a breakthrough development in solid state lighting, nanorod based LEDs have many potential advantages as compared to their 2 D thin film counterparts. In this paper, we review the recent developments of GaN nanorod growth, characterization, and related device applications based on GaN nanorods. The initial work on GaN nanorod growth focused on catalyst-assisted and catalyst-free statistical growth. The growth condition and growth mechanisms were extensively investigated and discussed. Doping of GaN nanorods, especially p-doping, was found to significantly influence the morphology of GaN nanorods. The large surface of 3 D GaN nanorods induces new optical and electrical properties, which normally can be neglected in layered structures. Recently, more controlled selective area growth of GaN nanorods was realized using patterned substrates both by metalorganic chemical vapor deposition (MOCVD) and by molecular beam epitaxy (MBE). Advanced structures, for example, photonic crystals and DBRs are meanwhile integrated in GaN nanorod structures. Based on the work of growth and characterization of GaN nanorods, GaN nanoLEDs were reported by several groups with different growth and processing methods. Core/shell nanoLED structures were also demonstrated, which could be potentially useful for future high efficient LED structures. In this paper, we will discuss recent developments in GaN nanorod technology, focusing on the potential advantages, but also discussing problems and open questions, which may impose obstacles during the future development of a GaN nanorod based LED technology.

BRENDA in 2013: integrated reactions, kinetic data, enzyme function data, improved disease classification: new options and contents in BRENDA

Abstract: The BRENDA (BRaunschweig ENzyme DAtabase) enzyme portal (http://www.brenda-enzymes.org) is the main information system of functional biochemical and molecular enzyme data and provides access to seven interconnected databases. BRENDA contains 2.7 million manually annotated data on enzyme occurrence, function, kinetics and molecular properties. Each entry is connected to a reference and the source organism. Enzyme ligands are stored with their structures and can be accessed via their names, synonyms or via a structure search. FRENDA (Full Reference ENzyme DAta) and AMENDA (Automatic Mining of ENzyme DAta) are based on text mining methods and represent a complete survey of PubMed abstracts with information on enzymes in different organisms, tissues or organelles. The supplemental database DRENDA provides more than 910 000 new EC number–disease relations in more than 510 000 references from automatic search and a classification of enzyme–disease-related information. KENDA (Kinetic ENzyme DAta), a new amendment extracts and displays kinetic values from PubMed abstracts. The integration of the EnzymeDetector offers an automatic comparison, evaluation and prediction of enzyme function annotations for prokaryotic genomes. The biochemical reaction database BKM-react contains non-redundant enzyme-catalysed and spontaneous reactions and was developed to facilitate and accelerate the construction of biochemical models.

Long-Wavelength Helimagnetic Order and Skyrmion Lattice Phase in Cu 2 OSeO 3

Abstract: We report a long-wavelength helimagnetic superstructure in bulk samples of the ferrimagnetic insulator Cu2OSeO3. The magnetic phase diagram associated with the helimagnetic modulation inferred from small-angle neutron scattering and magnetization measurements includes a skyrmion lattice phase and is strongly reminiscent of MnSi, FeGe, and Fe1-xCoxSi, i.e., binary isostructural siblings of Cu2OSeO3 that order helimagnetically. The temperature dependence of the specific heat of Cu2OSeO3 is characteristic of nearly critical spin fluctuations at the helimagnetic transition. This provides putative evidence for effective spin currents as the origin of enhancements of the magnetodielectric response instead of atomic displacements considered so far.

Industrial biotechnology of Pseudomonas putida and related species

Abstract: Since their discovery many decades ago, Pseudomonas putida and related subspecies have been intensively studied with regard to their potential application in industrial biotechnology. Today, these Gram-negative soil bacteria, traditionally known as well-performing xenobiotic degraders, are becoming efficient cell factories for various products of industrial relevance including a full range of unnatural chemicals. This development is strongly driven by systems biotechnology, integrating systems metabolic engineering approaches with novel concepts from bioprocess engineering, including novel reactor designs and renewable feedstocks.

Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami.

Abstract: We study the distance-dependent quenching of fluorescence due to a metallic nanoparticle in proximity of a fluorophore. In our single-molecule measurements, we achieve excellent control over structure and stoichiometry by using self-assembled DNA structures (DNA origami) as a breadboard where both the fluorophore and the 10 nm metallic nanoparticle are positioned with nanometer precision. The single-molecule spectroscopy method employed here reports on the co-localization of particle and dye, while fluorescence lifetime imaging is used to directly obtain the correlation of intensity and fluorescence lifetime for varying particle to dye distances. Our data can be well explained by exact calculations that include dipole-dipole orientation and distances. Fitting with a more practical model for nanosurface energy transfer yields 10.4 nm as the characteristic distance of 50% energy transfer. The use of DNA nanotechnology together with minimal sample usage by attaching the particles to the DNA origami directly on the microscope coverslip paves the way for more complex experiments exploiting dye-nanoparticle interactions.

Environmental factors affect Acidobacterial communities below the subgroup level in grassland and forest soils.

Abstract: In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and are physiologically active in situ. However, their individual functions and interactions with higher taxa in soil are still unknown. Here, potential effects of land use, soil properties, plant diversity, and soil nanofauna on acidobacterial community composition were studied by cultivation-independent methods in grassland and forest soils from three different regions in Germany. The analysis of 16S rRNA gene clone libraries representing all studied soils revealed that grassland soils were dominated by subgroup Gp6 and forest soils by subgroup Gp1 Acidobacteria. The analysis of a large number of sites (n = 57) by 16S rRNA gene fingerprinting methods (terminal restriction fragment length polymorphism [T-RFLP] and denaturing gradient gel electrophoresis [DGGE]) showed that Acidobacteria diversities differed between grassland and forest soils but also among the three different regions. Edaphic properties, such as pH, organic carbon, total nitrogen, C/N ratio, phosphorus, nitrate, ammonium, soil moisture, soil temperature, and soil respiration, had an impact on community composition as assessed by fingerprinting. However, interrelations with environmental parameters among subgroup terminal restriction fragments (T-RFs) differed significantly, e.g., different Gp1 T-RFs correlated positively or negatively with nitrogen content. Novel significant correlations of Acidobacteria subpopulations (i.e., individual populations within subgroups) with soil nanofauna and vascular plant diversity were revealed only by analysis of clone sequences. Thus, for detecting novel interrelations of environmental parameters with Acidobacteria, individual populations within subgroups have to be considered.

Consequences of customer loyalty to the loyalty program and to the company

Abstract: Gaining customer loyalty is an important goal of marketing, and loyalty programs are intended to help in reaching it. Research on loyalty programs suggests that customers differentiate between loyalty to a company and loyalty to a loyalty program, yet little is known about the consequences of these two types of loyalty. Therefore, our study intends to make two main contributions: (1) improving our understanding of the constructs "program loyalty" and "company loyalty", (2) investigating the relative impact of the two types of loyalty on preference, intention, and purchase behavior for the case of a multi-firm loyalty program. Results indicate that company loyalty influences a customer's choice to visit a particular provider and to prefer it over competitors, but it is not a strong predictor of purchase behavior. Conversely, program loyalty is a far more important driver of purchase behavior. This implies that company loyalty primarily attracts customers to a particular provider and program loyalty ensures that once inside the store, more money is spent.

Molecular and Cellular Characterization of the Biological Effects of Ruthenium(II) Complexes Incorporating 2-Pyridyl-2-pyrimidine-4-carboxylic Acid

Abstract: A great majority of the Ru complexes currently studied in anticancer research exert their antiproliferative activity, at least partially, through ligand exchange. In recent years, however, coordinatively saturated and substitutionally inert polypyridyl Ru(II) compounds have emerged as potential anticancer drug candidates. In this work, we present the synthesis and detailed characterization of two novel inert Ru(II) complexes, namely, [Ru(bipy)2(Cpp-NH-Hex-COOH)]2+ (2) and [Ru(dppz)2(CppH)]2+ (3) (bipy = 2,2′-bipyridine; CppH = 2-(2′-pyridyl)pyrimidine-4-carboxylic acid; Cpp-NH-Hex-COOH = 6-(2-(pyridin-2-yl)pyrimidine-4-carboxamido)hexanoic acid; dppz = dipyrido[3,2-a:2′,3′-c]phenazine). 3 is of particular interest as it was found to have IC50 values comparable to cisplatin, a benchmark standard in the field, on three cancer cell lines and a better activity on one cisplatin-resistant cell line than cisplatin itself. The mechanism of action of 3 was then investigated in detail and it could be demonstrated t...

Meetings Matter Effects of Team Meetings on Team and Organizational Success

Abstract: This study follows the idea that the key to understanding team meeting effectiveness lies in uncovering the microlevel interaction processes throughout the meeting. Ninety-two regular team meetings...

Planetesimal formation by sweep-up: how the bouncing barrier can be beneficial to growth

Abstract: Context. The formation of planetesimals is often accredited to the collisional sticking of dust grains. The exact process is unknown, as collisions between larger aggregates tend to lead to fragmentation or bouncing rather than sticking. Recent laboratory experiments have however made great progress in the understanding and mapping of the complex physics involved in dust collisions.Aims. We study the possibility of planetesimal formation using the results of the latest laboratory experiments, particularly by including the fragmentation with mass transfer effect, which might lead to growth even at high impact velocities.Methods. We present a new experimentally and physically motivated dust collision model capable of predicting the outcome of a collision between two particles of arbitrary mass and velocity. The new model includes a natural description of cratering and mass transfer, and provides a smooth transition from equal- to different-sized collisions. It is used together with a continuum dust-size evolution code, which is both fast in terms of execution time and able to resolve the dust at all sizes, allowing for all types of interactions to be studied without biases.Results. For the general dust population, we find that bouncing collisions prevent any growth above millimeter-sizes. However, if a small number of cm-sized particles are introduced, for example by either vertical mixing or radial drift, they can act as a catalyst and start to sweep up the smaller particles. At a distance of 3 AU, 100-m-sized bodies are formed on a timescale of 1 Myr. Conclusions. Direct growth of planetesimals might be a possibility thanks to a combination of the bouncing barrier and the fragmentation with mass transfer effect. The bouncing barrier is here even beneficial, as it prevents the growth of too many large particles that would otherwise only fragment among each other, and creates a reservoir of small particles that can be swept up by larger bodies. However, for this process to work, a few seeds of cm-size or larger have to be introduced.

An overview of the spectral induced polarization method for near-surface applications

Abstract: Over the last 15 years significant advancements in induced polarization (IP) research have taken place, particularly with respect to spectral IP (SIP), concerning the understanding of the mechanisms of the IP phenomenon, the conduction of accurate and broadband laboratory measurements, the modelling and inversion of IP data for imaging purposes and the increasing application of the method in near-surface investigations. We summarize here the current state of the science of the SIP method for near-surface applications and describe which aspects still represent open issues and should be the focus of future research efforts. Significant progress has been made over the last decade in the understanding of the microscopic mechanisms of IP; however, integrated mechanistic models involving different possible polarization processes at the grain/pore scale are still lacking. A prerequisite for the advances in the mechanistic understanding of IP was the development of improved laboratory instrumentation, which has led to a continuously growing data base of SIP measurements on various soil and rock samples. We summarize the experience of numerous experimental studies by formulating key recommendations for reliable SIP laboratory measurements. To make use of the established theoretical and empirical relationships between SIP characteristics and target petrophysical properties at the field scale, sophisticated forward modelling and inversion algorithms are needed. Considerable progress has also been made in this field, in particular with the development of complex resistivity algorithms allowing the modelling and inversion of IP data in the frequency domain. The ultimate goal for the future are algorithms and codes for the integral inversion of 3D, time-lapse and multi-frequency IP data, which defines a 5D inversion problem involving the dimensions space (for imaging), time (for monitoring) and frequency (for spectroscopy). We also offer guidelines for reliable and accurate measurements of IP spectra, which are essential for improved understanding of IP mechanisms and their links to physical, chemical and biological properties of interest. We believe that the SIP method offers potential for subsurface structure and process characterization, in particular in hydrogeophysical and biogeophysical studies.

Molecular Fractionation of Dissolved Organic Matter with Metal Salts

Abstract: Coagulation of dissolved organic matter (DOM) by hydrolyzing metals is an important environmental process with particular relevance, e.g., for the cycling of organic matter in metal-rich aquatic systems or the flocculation of organic matter in wastewater treatment plants. Often, a nonremovable fraction of DOM remains in solution even at low DOM/metal ratios. Because coagulation by metals results from interactions with functional groups, we hypothesize that noncoagulating fractions have a distinct molecular composition. To test the hypothesis, we analyzed peat-derived dissolved organic matter remaining in solution after mixing with salts of Ca, Al, and Fe using 15 T Electrospray Ionization Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (ESI-FT-ICR-MS). Addition of metals resulted in a net removal of DOM. Also a reduction of molecular diversity was observed, as the number of peaks from the ESI-FT-ICR-MS spectra decreased. At DOM/metal ratios of ∼9 Ca did not show any preference for distinct mol...

CheapBFT: resource-efficient byzantine fault tolerance

Abstract: One of the main reasons why Byzantine fault-tolerant (BFT) systems are not widely used lies in their high resource consumption: 3f+1 replicas are necessary to tolerate only f faults. Recent works have been able to reduce the minimum number of replicas to 2f+1 by relying on a trusted subsystem that prevents a replica from making conflicting statements to other replicas without being detected. Nevertheless, having been designed with the focus on fault handling, these systems still employ a majority of replicas during normal-case operation for seemingly redundant work. Furthermore, the trusted subsystems available trade off performance for security; that is, they either achieve high throughput or they come with a small trusted computing base.This paper presents CheapBFT, a BFT system that, for the first time, tolerates that all but one of the replicas active in normal-case operation become faulty. CheapBFT runs a composite agreement protocol and exploits passive replication to save resources; in the absence of faults, it requires that only f+1 replicas actively agree on client requests and execute them. In case of suspected faulty behavior, CheapBFT triggers a transition protocol that activates f extra passive replicas and brings all non-faulty replicas into a consistent state again. This approach, for example, allows the system to safely switch to another, more resilient agreement protocol. CheapBFT relies on an FPGA-based trusted subsystem for the authentication of protocol messages that provides high performance and comprises a small trusted computing base.

Simplified evaporation method for determining soil hydraulic properties: a reinvestigation of linearization errors

Abstract: Accurate knowledge of the soil hydraulic properties is a prerequisite for reliable modelling of soil water dynamics. As a consequence, many methods have been developed to derive these constitutive relationships either under field or laboratory conditions. Among these methods, the simplified evaporation method conducted on soil samples in the laboratory has found widespread use and application, mainly due to its relative ease of implementation and its straightforward evaluation of the experimental data. This method, however, relies on various simplifying assumptions. A common approach to assess the validity of these assumptions and to explore potential linearization errors associated with them is the use of synthetic data. In the past, such synthetic data were generated using rather simplistic models considering liquid water flow in capillaries only. In this study, we reinvestigated the accuracy of the simplified evaporation method using a more realistic process description of evaporative drying of the soil sample, including both liquid water flow in capillaries and films, as well as isothermal water vapour diffusion. In contrast to previous results reported in the literature, our results show that the simplifying assumptions used to evaluate the experimental data may result in biased estimates of the soil hydraulic properties, particularly for coarse textured soils. The bias typically increased progressively during stage-two evaporation, which is characterized by the development of a dry surface layer in which water flow is dominated by diffusion of water vapour, resulting in strongly nonlinear pressure head and water content profiles. We investigated various strategies for correcting for this bias caused by simplifying assumptions.

First large-scale DNA barcoding assessment of reptiles in the biodiversity hotspot of Madagascar, based on newly designed COI primers.

Abstract: Background DNA barcoding of non-avian reptiles based on the cytochrome oxidase subunit I (COI) gene is still in a very early stage, mainly due to technical problems. Using a newly developed set of reptile-specific primers for COI we present the first comprehensive study targeting the entire reptile fauna of the fourth-largest island in the world, the biodiversity hotspot of Madagascar. Methodology/Principal Findings Representatives of the majority of Madagascan non-avian reptile species (including Squamata and Testudines) were sampled and successfully DNA barcoded. The new primer pair achieved a constantly high success rate (72.7–100%) for most squamates. More than 250 species of reptiles (out of the 393 described ones; representing around 64% of the known diversity of species) were barcoded. The average interspecific genetic distance within families ranged from a low of 13.4% in the Boidae to a high of 29.8% in the Gekkonidae. Using the average genetic divergence between sister species as a threshold, 41–48 new candidate (undescribed) species were identified. Simulations were used to evaluate the performance of DNA barcoding as a function of completeness of taxon sampling and fragment length. Compared with available multi-gene phylogenies, DNA barcoding correctly assigned most samples to species, genus and family with high confidence and the analysis of fewer taxa resulted in an increased number of well supported lineages. Shorter marker-lengths generally decreased the number of well supported nodes, but even mini-barcodes of 100 bp correctly assigned many samples to genus and family. Conclusions/Significance The new protocols might help to promote DNA barcoding of reptiles and the established library of reference DNA barcodes will facilitate the molecular identification of Madagascan reptiles. Our results might be useful to easily recognize undescribed diversity (i.e. novel taxa), to resolve taxonomic problems, and to monitor the international pet trade without specialized expert knowledge.

Color and Albedo Heterogeneity of Vesta from Dawn

Abstract: Multispectral images (0.44 to 0.98 μm) of asteroid (4) Vesta obtained by the Dawn Framing Cameras reveal global color variations that uncover and help understand the north-south hemispherical dichotomy. The signature of deep lithologies excavated during the formation of the Rheasilvia basin on the south pole has been preserved on the surface. Color variations (band depth, spectral slope, and eucrite-diogenite abundance) clearly correlate with distinct compositional units. Vesta displays the greatest variation of geometric albedo (0.10 to 0.67) of any asteroid yet observed. Four distinct color units are recognized that chronicle processes—including impact excavation, mass wasting, and space weathering—that shaped the asteroid’s surface. Vesta’s color and photometric diversity are indicative of its status as a preserved, differentiated protoplanet.

A basic tutorial on cyclic voltammetry for the investigation of electroactive microbial biofilms.

Abstract: Electroactive microbial biofilms and the microorganisms embedded therein are not only of crucial fundamental interest because they play an important role in redox cycles that occur in nature, they are also attracting increasing attention as key component of microbial bioelectrochemcial systems (BES) In these systems, interconversion of chemical and electrical energy and the associated exchange of electrons between living microbial cells and solid electrodes take place The fascinating prospects and promise of BES technology have considerably increased the research on electroactive microbial biofilms over recent years As a consequence, the research community is truly multifaceted, with backgrounds and interests ranging from molecular biology, via chemistry, to engineering One of the most-important and most-widespread applied electrochemical techniques is cyclic voltammetry (CV) This Focus Review illustrates the power of this electrochemical technique and the versatility of the information that can be gained by its application for the electrochemical freshman This Review will also pinpoint hurdles in using this technique, especially for the non-electrochemist, and the limitations of present models for data analysis Because it aims to be a basic introduction, this Review will not discuss the latest intricacies in the field

Layered corrugated electrode macrostructures boost microbial bioelectrocatalysis

Abstract: The future success of microbial bioelectrochemical systems like microbial fuel cells inevitably depends on the increase of their performance at decreasing material costs. One of the key elements and a research priority is the biofuel cell anode. Here we propose layered corrugated carbon (LCC) as an inexpensive but high performance electrode material produced from the carbonization of one of the most abundant packing materials of our society: corrugated cardboard. In the base configuration of one corrugated layer the projected current density of LCC already reaches 70 A m−2. Increasing the number of corrugated layers increases the current density linearly. Thus, 200 A m−2 are achieved at three and 390 A m−2 at six corrugated layers. These current density values, which were confirmed by experiments in two independent laboratories, represent a performance increase of above one order of magnitude compared to the current state of research.