Jewish Hospital

About: Jewish Hospital is a healthcare organization based out in Cincinnati, Ohio, United States. It is known for research contribution in the topics: Antigen & Population. The organization has 3881 authors who have published 3414 publications receiving 123044 citations.

Safety and Clinical Outcomes of Rituximab Treatment in Patients with Multiple Sclerosis and Neuromyelitis Optica: Experience from a National Online Registry (GRAID)

Abstract: Introduction Multiple sclerosis (MS) is an immune-mediated disease. Over the last decades therapeutic options have broadened tremendously. Nevertheless, various therapeutic agents, e.g., rituximab, are currently used in the treatment of MS off label. Disease or health registries are useful methods to collect information about off-label treatments. The German registry for autoimmune disease (GRAID) is a multicenter, retrospective, non-interventional database of patients with various autoimmune diseases.

Indole reactions in bacteria

Abstract: Kitasato's (1889) discovery that Escherichia coli could be distinguished from Klebsiella species (Aerobacter aerogenes) by virtue of its ability to produce indole initiated the detection of indole production as an accepted analytical tool for the bacteriologist. The use of p-dimethylaminobenzaldehyde for this purpose, first applied by B6hme (1906), modified by Kovacs (1928), and most recently refined by Gadebusch and Gabriel (1956), has become the accepted method for the detection of this metabolite in cultures. Thus, Edwards and Ewing (1955) and Kauffmann (1954) recommend the use of the simple Kovacs' test (p-DAB, direct). The Manual of Microbiological Mlethods (1957) indicates Gore's (1921) modification is more specific than the aforementioned Kovacs' test. Although the relationship between tryptophan and indole was obvious to investigators as early as 1912 (Zipfel), the majority of workers were unable to enhance indole production by the addition of tryptophan to the medium (Kulp, 1925). Wood et al. (1947) introduced tryptophan as an integral part of the medium for the quantitative estimation of tryptophanase. Since p-dimethylaminobenzaldehyde reacts not only with indole but also with a and 3 substituted pyrroles, investigators advised distillation of cultures to separate the more volatile indole from related metabolites (Fellers and Clough, 1925; Gore, 1921). Wood et al. (1947) extracted indole with toluene prior to its treatment with p-dimethylaminobenzaldehyde. Gallop and Seifter (1958, unpublished data) discovered recently that alkaline hydroxylamine reacts specifically with unsubstituted pyrroles and indole to form products which yield colored complexes in acidic media. In view of the lack of specificity of the widely used direct p-dimethylaminobenzaldehyde test, a study comparing that determination with another modified Ehrlich's test (extracted pdimethylaminobenzaldehyde) and with the hydroxylamine reaction was made. MATERIALS AND METHODS

The inhibitory effect of salicylate on the acetylation of human albumin by acetylsalicylic acid.

Abstract: The transacetylation reaction between aspirin and human serum albumin (HSA) is partially inhibited by pharmacologic concentrations of salicylate anion. This inhibition was noted with respect to the inhibition of enhanced acetrizoate binding and by autoradiography of peptide maps of aspirin-acetylated HSA. The acetylation of a single lysine residue in a peptide 7“A” has been found to be responsible for the previously reported, enhanced binding of acetrizoate by aspirin-altered albumin. At least 5 other peptides are also acetylated, but they do not influence acetrizoate binding. The results are discussed in view of the unique pharmacologic activities of aspirin, and also, with respect to biologic reactions ascribed to aspirin therapy.

In vitro maturation of precursors of 5S ribosomal RNA from Bacillus subtilis.

Abstract: THE ribosomal RNA (rRNA) molecules both of prokaryotes and of eukaryotes are not the immediate products of DNA transcription. Rather, precursors of the mature RNA species undergo a series of nucleotide modifications and/or cleavage events1,2. Only limited information is available concerning the mechanics of and rationale for post-transcriptional tailoring of RNA because of the large sizes of the precursor molecules and consequent difficulty in evaluating their nucleotide sequences.