Jewish Hospital

About: Jewish Hospital is a healthcare organization based out in Cincinnati, Ohio, United States. It is known for research contribution in the topics: Antigen & Population. The organization has 3881 authors who have published 3414 publications receiving 123044 citations.

Immunological identity of insulin from various species1

Abstract: THE STRIKING similarity in the physiological activity and in the chemical analysis (I) of purified extracts of insulin derived from various animal sources prompted further work in an attempt to demonstrate more fully the identity of insulin prepared from different species. An immunological approach has been used since it already has been demonstrated that insulin is active as an antigen, producing complement fixation antibodies (2) and anaphylactic antibodies(3).Using the SchultS-Dale anaphylactic response, Lewis (3) demonstrated that typical reactions indicative of anaphylaxis may be produced by sensitising pigs with insulin extracted from the pancreas of one species and then using, as the shocking antigen, insulin produced from an entirely different species. The report of Bernstein, Kirsner, and Turner (4) on anaphylaxis in guinea pigs, using insulin as the antigen, is scarcely decisive in settling the point in question since the same type of insulin was used both for sensitisation and for shocking purp...