Attomolar DNA detection with chiral nanorod assemblies
TLDR
It is demonstrated that gold nanorods assembled by the polymerase chain reaction into DNA-bridged chiral systems have promising analytical applications and may be particularly useful for biological analytes larger than 2–5 nm which are difficult to detect by methods based on plasmon coupling and ‘hot spots’.Abstract:
Nanoscale plasmonic assemblies display exceptionally strong chiral optical activity. So far, their structural design was primarily driven by challenges related to metamaterials whose practical applications are remote. Here we demonstrate that gold nanorods assembled by the polymerase chain reaction into DNA-bridged chiral systems have promising analytical applications. The chiroplasmonic activity of side-by-side assembled patterns is attributed to a 7-9 degree twist between the nanorod axes. This results in a strong polarization rotation that matches theoretical expectations. The amplitude of the bisignate 'wave' in the circular dichroism spectra of side-by-side assemblies demonstrates excellent linearity with the amount of target DNA. The limit of detection for DNA using side-by-side assemblies is as low as 3.7 aM. This chiroplasmonic method may be particularly useful for biological analytes larger than 2-5 nm which are difficult to detect by methods based on plasmon coupling and 'hot spots'. Circular polarization increases for inter-nanorod gaps between 2 and 20 nm when plasmonic coupling rapidly decreases. Reaching the attomolar limit of detection for simple and reliable bioanalysis of oligonucleotides may have a crucial role in DNA biomarker detection for early diagnostics of different diseases, forensics and environmental monitoring.read more
Citations
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References
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Journal ArticleDOI
Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method
TL;DR: In this article, a method was used for preparing gold NRs with aspect ratios ranging from 1.5 to 4.5 for which the surface plasmon absorption maxima are between 600 and 1300 nm.
Journal ArticleDOI
Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles
TL;DR: A highly selective, colorimetric polynucleotide detection method based on mercaptoalkyloligonucleotide-modified gold nanoparticle probes is reported, which can detect about 10 femtomoles of an oligonucleotide.
Journal ArticleDOI
DNA-based self-assembly of chiral plasmonic nanostructures with tailored optical response
Anton Kuzyk,Robert D. Schreiber,Zhiyuan Fan,Günther Pardatscher,Eva-Maria Roller,Alexander Högele,Friedrich C. Simmel,Alexander O. Govorov,Tim Liedl +8 more
TL;DR: It is shown that DNA origami enables the high-yield production of plasmonic structures that contain nanoparticles arranged in nanometre-scale helices, and it is found that the structures in solution exhibit defined circular dichroism and optical rotatory dispersion effects at visible wavelengths that originate from the collective plAsmon–plasmon interactions of the nanoparticles positioned with an accuracy better than two nanometres.
Journal ArticleDOI
A Group-IV Ferromagnetic Semiconductor: MnxGe1−x
Yun Daniel Park,Aubrey T. Hanbicki,Steven C. Erwin,C. S. Hellberg,James M. Sullivan,J. E. Mattson,T. Ambrose,A. Wilson,George Spanos,Berend T. Jonker +9 more
TL;DR: Calculated spin interactions and percolation theory predict transition temperatures larger than measured, consistent with the observed suppression of magnetically active Mn atoms and hole concentration.
Journal ArticleDOI
Array-based electrical detection of DNA with nanoparticle probes
TL;DR: Using this method, target DNA is detected at concentrations as low as 500 femtomolar with a point mutation selectivity factor of ∼ 100,000:1 and an unusual salt concentration–dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash.