Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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RNAs as Candidate Diagnostic and Prognostic Markers of Prostate Cancer—From Cell Line Models to Liquid Biopsies
Marvin Lim,Anne-Marie Baird,Anne-Marie Baird,John Aird,John Greene,Dhruv Kapoor,Steven G. Gray,Steven G. Gray,Ray McDermott,Stephen P. Finn +9 more
TL;DR: This review focuses on the evidence for the role of RNAs in prostate cancer, and their use as diagnostic and prognostic markers, and targets for treatment in this disease.
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Identification, characterization, and functional investigation of circular RNAs in subventricular zone of adult rat brain.
TL;DR: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that the SVZ‐specific circ RNAs have close relationship with the regulation of NSC expansion and NSC‐niche interaction, while the other differentially expressed circRNAs might be involved in neural cellular construction and nerve system function.
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Hsa_circ_0107593 Suppresses the Progression of Cervical Cancer via Sponging hsa-miR-20a-5p/93-5p/106b-5p.
Wenyan Liao,Jun He,Cyrollah Disoma,Yi Hu,Junhua Li,Guodong Chen,Ying Sheng,Xinyu Cai,Chuanfu Li,Kai Cheng,Chunfen Yang,Yongxi Qin,Dong Han,Wu Wen,Chengming Ding,Mujun Li +15 more
TL;DR: In this paper, the role and molecular mechanism of hsa_circ_0107593 in cervical cancer were demonstrated, where the authors used qRT-PCR and dual-luciferase reporter assay to determine the expression of the circRNAs and three miRNAs in paired CC tissues (tumor tissue vs. adjacent normal cervical tissue), and human normal cervical epithelial immortalized cell line.
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RNA-mediated genome rearrangement: hypotheses and evidence.
Wenwen Fang,Laura F. Landweber +1 more
TL;DR: This work has shown how chromatin prevents genomic rearrangements by blockingocus colocalization induced by transcription factor binding, and also investigates the role of EMT in this process.
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Non-coding RNAs: the extensive and interactive regulators of the blood-brain barrier permeability
TL;DR: In this article, the authors summarized the recent research progress on miRNA, lncRNA, and circRNA roles regulating the BBB permeability in different CNS diseases, including Alzheimer's disease, multiple sclerosis, traumatic brain injury, stroke, meningitis, and brain cancers.
References
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Journal ArticleDOI
A coding-independent function of gene and pseudogene mRNAs regulates tumour biology
Laura Poliseno,Leonardo Salmena,Jiangwen Zhang,Brett S. Carver,William J. Haveman,Pier Paolo Pandolfi +5 more
TL;DR: It is found that PTENP1 is biologically active as it can regulate cellular levels of PTEN and exert a growth-suppressive role, and this analysis extended to other cancer-related genes that possess pseudogenes, and revealed a non-coding function for mRNAs.
Journal ArticleDOI
Circular transcripts of the testis-determining gene Sry in adult mouse testis
Blanche Capel,Amanda Swain,Silvia K. Nicolis,Adam Hacker,Michael A. Walter,Peter Koopman,Peter N. Goodfellow,Robin Lovell-Badge +7 more
TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
Journal ArticleDOI
Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
Thomas B. Hansen,Erik D Wiklund,Erik D Wiklund,Jesper B. Bramsen,Sune B. Villadsen,Aaron L. Statham,Susan J. Clark,Jørgen Kjems +7 more
TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
Journal ArticleDOI
Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.