Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Progress and prospects of noncoding RNAs in insects
TL;DR: In this article, the authors reviewed the research progress of four kinds of ncRNAs, including microRNA (miRNA), Piwi-interacting RNA (piRNA), circular RNA (circRNA), and long noncoding RNA (lncRNA) in insects.
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Dynamic ASXL1 Exon Skipping and Alternative Circular Splicing in Single Human Cells.
Winston Koh,Veronica Gonzalez,Sivaraman Natarajan,Robert Carter,Patrick O. Brown,Charles Gawad +5 more
TL;DR: A combination of targeted deletion, high-resolution splicing detection, and single-cell sequencing revealed a strong preference for either the linear or circular ASXL1 isoforms in each cell, and suggests that standard methods overestimate circRNA abundance.
Journal ArticleDOI
Reinventing the Wheel: Synthetic Circular RNAs for Mammalian Cell Engineering.
TL;DR: Reports of reported uses of synthetic circular RNAs are highlighted and methods for generating these molecules are described, which show the promise in the production of recombinant proteins and as RNA-based therapies.
Journal ArticleDOI
CircRNA-Mediated Regulation of Angiogenesis: A New Chapter in Cancer Biology.
Shaotao Jiang,Rongdang Fu,Jiewei Shi,Huijie Wu,Jialuo Mai,Xuefeng Hua,Huan Chen,Jie Liu,Minqiang Lu,Ning Li +9 more
TL;DR: This review investigated circRNAs in tumor angiogenesis from multiple perspectives, including its upstream and downstream factors, and believes thatcircRNAs have natural advantages and great potential for the diagnosis and treatment of tumors.
Journal ArticleDOI
CircRNAs Are Here to Stay: A Perspective on the MLL Recombinome.
Anna Dal Molin,Silvia Bresolin,Enrico Gaffo,Caterina Tretti,Elena Boldrin,Lueder H. Meyer,Paola Guglielmelli,Alessandro M. Vannucchi,Geertruij te Kronnie,Stefania Bortoluzzi +9 more
TL;DR: Evidence is provided that rearrangements of MLL and three of the main translocation partner genes can impact circRNA expression, supported also by preliminary observations in leukemic cells, which underpins the view that circRNAs are worthwhile to be considered when studying MLLre leukemias.
References
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TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
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Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
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TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
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Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.