Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Understanding Long Noncoding RNA and Chromatin Interactions: What We Know So Far.
TL;DR: How technological advancements contributed in characterizing chromatin associated lncRNAs is reviewed, and the potential mechanisms by which chromatinassociated lnc RNAs execute their functions are discussed.
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Functional analysis of long noncoding RNAs in development and disease.
Ling-Ling Chen,Jing Crystal Zhao +1 more
TL;DR: An overview of different lnc RNAs families is provided, methodologies available to study lncRNA-protein and lnc RNA-DNA interactions systematically are described, and well-studied lncRNAs are used as examples to illustrate their functional importance during normal development and in disease states.
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Circular RNA Expression Profiling Identifies Prostate Cancer- Specific circRNAs in Prostate Cancer
Qianlin Xia,Qianlin Xia,Tao Ding,Guihong Zhang,Zehuan Li,Ling Zeng,Yanjun Zhu,Jianming Guo,Jun Hou,Tongyu Zhu,Jianghua Zheng,Jin Wang +11 more
TL;DR: The results demonstrated that differentially expressed circRNAs (circ_006 2019 and circ_0057558) and host gene SLC19A1 of circ_0062019 could be used as potential novel biomarkers for prostate cancer.
Journal ArticleDOI
Circular RNAs are differentially expressed in prostate cancer and are potentially associated with resistance to enzalutamide.
John Greene,Anne-Marie Baird,Orla Casey,Lauren Brady,Gordon Blackshields,Marvin Lim,Marvin Lim,Odharnaith O'Brien,Steven G. Gray,Raymond S. McDermott,Raymond S. McDermott,Stephen P. Finn +11 more
TL;DR: This is one of the first studies to profile and demonstrate discrete circRNAs expression patterns in an enzalutamide resistant cell line model of prostate cancer, and suggests that hsa_circ_0004870, through RBM39, may play a critical role in the development of enzyme resistance in CRPC.
Book ChapterDOI
Noncoding RNAs: New Players in Cancers.
TL;DR: This chapter will review the current knowledge of the ncRNA field, discussing the genomic context, biological functions, and mechanisms of action of miRNAs, lnc RNAs, and circRNAs.
References
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TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
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Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
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TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
Journal ArticleDOI
Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.