Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Circular RNAs: Biogenesis, Function, and a Role as Possible Cancer Biomarkers
TL;DR: The potential of circRNAs to be effectively used as novel cancer diagnostic and prognostic biomarkers is addressed and they are regarded as promising novel biomarkers in cancer.
Journal ArticleDOI
Circular RNAs: Novel Regulators of Neuronal Development.
TL;DR: Evidence from analysis of some circRNA molecules suggests that they could substantially contribute to the regulation of gene expression, particularly in architecturally complex and polarized cells such as neurons.
Journal ArticleDOI
Genome-wide profiling of Sus scrofa circular RNAs across nine organs and three developmental stages.
TL;DR: This work comprehensively analysed circRNAs in nine organs and three skeletal muscles of Guizhou miniature pig, a widely used biomedical model animal, and constructed the first public S. scrofa circRNA database, allowing researchers to query comprehensive annotation, expression, and regulatory networks ofcircRNAs.
Journal ArticleDOI
Circular RNA circRHOBTB3 acts as a sponge for miR-654-3p inhibiting gastric cancer growth.
TL;DR: It is revealed that circRHOBTB3 might function as competing endogenous RNA (ceRNA) for miR-654-3p, which could contribute to growth inhibition of GC through activating p21 signaling pathway.
Journal ArticleDOI
The decade of exosomal long RNA species: an emerging cancer antagonist
Ruihao Zhou,Kaddie Kwok Chen,Kaddie Kwok Chen,Jingtao Zhang,Bufan Xiao,Zhaohao Huang,Cheng Ju,Jun Sun,Feifei Zhang,Xiao Bin Lv,Guofu Huang +10 more
TL;DR: The role of exosomal long RNA species, which is comprised of mRNA, lncRNA, and circRNA, in tumor formation and progression is analyzed, with an emphasis on their potential as future diagnostic biomarkers and treatment vectors in cancer biology.
References
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TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
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Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
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TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
Journal ArticleDOI
Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.