Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Journal ArticleDOI
Molecular roles and function of circular RNAs in eukaryotic cells.
TL;DR: It is described how circRNAs impact gene expression of their host gene locus by affecting transcriptional initiation and elongation or splicing, and how they partake in controlling the function of other molecules, for example by interacting with microRNAs and proteins.
Journal ArticleDOI
A multi-split mapping algorithm for circular RNA, splicing, trans-splicing and fusion detection
Steve Hoffmann,Christian Otto,Gero Doose,Andrea Tanzer,David Langenberger,Sabina Christ,Manfred Kunz,Lesca M. Holdt,Lesca M. Holdt,Daniel Teupser,Daniel Teupser,Jörg Hackermüller,Jörg Hackermüller,Peter F. Stadler +13 more
TL;DR: In this paper, an unbiased algorithm was proposed to detect splice junctions from single-end cDNA sequences. But this method is not suitable for reads with multiple splits, trans-splicing and circular products.
Journal ArticleDOI
A circular RNA promotes tumorigenesis by inducing c-myc nuclear translocation.
Qi Yang,William W. Du,William W. Du,Nan Wu,Nan Wu,Weining Yang,Faryal Mehwish Awan,Faryal Mehwish Awan,Ling Fang,Ling Fang,Ling Fang,Jian Ma,Jian Ma,Xiangmin Li,Xiangmin Li,Yan Zeng,Yan Zeng,Zhenguo Yang,Zhenguo Yang,Jun Dong,Jun Dong,Azam Khorshidi,Azam Khorshidi,Burton B. Yang,Burton B. Yang +24 more
TL;DR: A novel function of circRNAs in tumorigenesis is revealed, and this subclass of noncoding RNAs may represent a potential target in cancer therapy.
Journal ArticleDOI
CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630.
TL;DR: The results suggest that circRNA_100269 and miR-630 comprise a novel pathway that regulates proliferation of GC cells.
Journal ArticleDOI
Circular RNA has_circ_0067934 is upregulated in esophageal squamous cell carcinoma and promoted proliferation.
Wenjia Xia,Mantang Qiu,Rui Chen,Rui Chen,Siwei Wang,Xuechun Leng,Jie Wang,Youtao Xu,Jingwen Hu,Gaochao Dong,Lin Xu,Rong Yin +11 more
TL;DR: It is found that hsa_circ_0067934 was significantly overexpressed in ESCC tissues compared with paired adjacent normal tissues and the high expression level was associated with poor differentiation, which inhibited the proliferation and migration of ESCC cells and blocked cell cycle progression.
References
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Journal ArticleDOI
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Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.