Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
More filters
Journal ArticleDOI
An Emerging Role for Circular RNAs in Osteoarthritis
Chen Xi Yu,Shui Sun +1 more
TL;DR: The biogenesis, characteristics, and biofunctions of circRNAs are delineated, and the role ofcircRNAs in the occurrence and progression OA is focused on.
Journal ArticleDOI
How are circRNAs translated by non-canonical initiation mechanisms?
Leila H Diallo,Florence Tatin,Florian David,Anne-Claire Godet,Audrey Zamora,Anne-Catherine Prats,Barbara Garmy-Susini,Eric Lacazette +7 more
TL;DR: Findings link circRNA translation to cellular response to stress conditions, raising new enquiries about the regulation of circRNA expression under stress conditions and their functions.
Journal ArticleDOI
Microarray expression profile analysis of circular RNAs in pancreatic cancer.
Shixiang Guo,Xuejun Xu,Yongsheng Ouyang,Yunchao Wang,Jiali Yang,Liangyu Yin,Jiayun Ge,Huaizhi Wang +7 more
TL;DR: Investigation of the expression profile of circRNAs in 20 pancreatic cancer tissues and corresponding paracancerous tissues using arraystar human circRNA array analysis, high-throughput circRNA microarray, bioinformatic analysis and reverse transcription-quantitative polymerase chain reaction revealed that circ RNAs expression was visibly diverse in pancreatic Cancer.
Journal ArticleDOI
Circ-Foxo3 is positively associated with the Foxo3 gene and leads to better prognosis of acute myeloid leukemia patients
Jiao Zhou,Ling-Yu Zhou,Xi Tang,Jing Zhang,Ling-Ling Zhai,Yun Yun Yi,Jing Yi,Jiang Lin,Jun Qian,Zhao-Qun Deng +9 more
TL;DR: It is found that low expression of circ-Foxo3 and Foxo3 were frequent in AML patients, and patients with high expression of Foxe3 often had a trend of better prognosis.
Journal ArticleDOI
Circular RNA expression and function in the brain.
Shobana Sekar,Winnie S. Liang +1 more
TL;DR: Recent research on circRNA expression in the brain is reviewed, the proposed functions of circRNAs are described, and the role ofcircRNAs in neurological diseases is evaluated.
References
More filters
Journal ArticleDOI
A coding-independent function of gene and pseudogene mRNAs regulates tumour biology
Laura Poliseno,Leonardo Salmena,Jiangwen Zhang,Brett S. Carver,William J. Haveman,Pier Paolo Pandolfi +5 more
TL;DR: It is found that PTENP1 is biologically active as it can regulate cellular levels of PTEN and exert a growth-suppressive role, and this analysis extended to other cancer-related genes that possess pseudogenes, and revealed a non-coding function for mRNAs.
Journal ArticleDOI
Circular transcripts of the testis-determining gene Sry in adult mouse testis
Blanche Capel,Amanda Swain,Silvia K. Nicolis,Adam Hacker,Michael A. Walter,Peter Koopman,Peter N. Goodfellow,Robin Lovell-Badge +7 more
TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
Journal ArticleDOI
Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
Thomas B. Hansen,Erik D Wiklund,Erik D Wiklund,Jesper B. Bramsen,Sune B. Villadsen,Aaron L. Statham,Susan J. Clark,Jørgen Kjems +7 more
TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
Journal ArticleDOI
Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.