Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
More filters
Journal ArticleDOI
The Role of microRNAs, Long Non-coding RNAs, and Circular RNAs in Cervical Cancer.
Maria Lina Tornesello,Raffaella Faraonio,Luigi Buonaguro,Clorinda Annunziata,Noemy Starita,Andrea Cerasuolo,Francesca Pezzuto,Anna Lucia Tornesello,Franco M. Buonaguro +8 more
TL;DR: Current literature on the complex interplay between miRNAs, lnc RNAs, and circRNAs and their role in cervical neoplasia is summarized.
Journal ArticleDOI
Chemical Modifications in the Life of an mRNA Transcript.
Sigrid Nachtergaele,Chuan He +1 more
TL;DR: Investigations over the past eight years of chemical modifications on messenger RNA have revealed a new level of posttranscriptional gene regulation in eukaryotes, and m6A methylation provides a rapid mechanism for coordinated transcriptome processing and turnover that is important in embryonic development and cell differentiation.
Journal ArticleDOI
Circular RNAs as Therapeutic Agents and Targets.
TL;DR: Reports, where manipulating circRNA abundance has been shown to have therapeutic value in animal disease models in vivo, are described, with a focus on cardiovascular disease (CVD).
Journal ArticleDOI
The bioinformatics toolbox for circRNA discovery and analysis.
TL;DR: This review collected about 100 circRNA-associated bioinformatics tools and summarized their current attributes and capabilities and performed network analysis and text mining on circRNA tool publications in order to reveal trends in their ongoing development.
Journal ArticleDOI
Silencing CDR1as inhibits colorectal cancer progression through regulating microRNA-7.
Wentao Tang,Meiling Ji,Guodong He,Liangliang Yang,Zhengchuan Niu,Mi Jian,Ye Wei,Li Ren,Jianmin Xu +8 more
TL;DR: Downregulation of CDR1as suppressed CRC cell proliferation and invasion and increased microRNA-7 (miR-7) expression, and Mechanistic studies demonstrated that CDR 1as silencing suppressed EGFR and IGF-1R expression, which could be partially blocked by the miR- 7 inhibitor.
References
More filters
Journal ArticleDOI
A coding-independent function of gene and pseudogene mRNAs regulates tumour biology
Laura Poliseno,Leonardo Salmena,Jiangwen Zhang,Brett S. Carver,William J. Haveman,Pier Paolo Pandolfi +5 more
TL;DR: It is found that PTENP1 is biologically active as it can regulate cellular levels of PTEN and exert a growth-suppressive role, and this analysis extended to other cancer-related genes that possess pseudogenes, and revealed a non-coding function for mRNAs.
Journal ArticleDOI
Circular transcripts of the testis-determining gene Sry in adult mouse testis
Blanche Capel,Amanda Swain,Silvia K. Nicolis,Adam Hacker,Michael A. Walter,Peter Koopman,Peter N. Goodfellow,Robin Lovell-Badge +7 more
TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
Journal ArticleDOI
Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
Thomas B. Hansen,Erik D Wiklund,Erik D Wiklund,Jesper B. Bramsen,Sune B. Villadsen,Aaron L. Statham,Susan J. Clark,Jørgen Kjems +7 more
TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
Journal ArticleDOI
Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.