Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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circRNA_100290 plays a role in oral cancer by functioning as a sponge of the miR-29 family.
TL;DR: It is concluded that circRNA_100290 may function as a competing endogenous RNA to regulate CDK6 expression through sponging up miR-29b family members and may be a potential target for OSCC therapy.
Journal ArticleDOI
Circular RNA - New member of noncoding RNA with novel functions.
TL;DR: The review summarizes the current hypotheses for the models of circRNA biosynthesis including the direct interaction between upstream and downstream introns and lariat-driven circularization and the potential roles of circRNAs in neural system, cardiovascular system as well as cancers.
Journal ArticleDOI
The emerging landscape of circular RNA in life processes
TL;DR: The current understanding of circRNA classification, function and significance in physiological and pathological processes is reviewed, and future research will increase the understanding of the regulation and function of these novel molecules.
Journal ArticleDOI
A 360° view of circular RNAs: From biogenesis to functions
TL;DR: Circular RNAs represent a large class of RNA molecules that are tightly regulated, and it is becoming increasingly clear that they likely impact many biological processes.
Journal ArticleDOI
Transcriptome-wide investigation of circular RNAs in rice.
Tingting Lu,Lingling Cui,Yan Zhou,Chuanrang Zhu,Danlin Fan,Hao Gong,Qiang Zhao,Congcong Zhou,Yan Zhao,Danfeng Lu,Jianghong Luo,Yongchun Wang,Qilin Tian,Qi Feng,Tao Huang,Bin Han +15 more
TL;DR: Analysis of deep sequencing and computational analysis of ssRNA-seq data reveal the prevalence of circRNAs in rice and provide new biological insights into rice circ RNAs.
References
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Circular transcripts of the testis-determining gene Sry in adult mouse testis
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TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
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Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
Thomas B. Hansen,Erik D Wiklund,Erik D Wiklund,Jesper B. Bramsen,Sune B. Villadsen,Aaron L. Statham,Susan J. Clark,Jørgen Kjems +7 more
TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
Journal ArticleDOI
Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.