Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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A Schwann cell-enriched circular RNA circ-Ankib1 regulates Schwann cell proliferation following peripheral nerve injury.
TL;DR: A crucial role for circRNAs is revealed in regulating proliferation of SCs involved in sciatic nerve regeneration; as such,circRNAs may serve as a potential therapeutic avenue for nerve injury repair.
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Profiling of differentially expressed circular RNAs in peripheral blood mononuclear cells from Alzheimer’s disease patients
Yanxin Li,Zhanyun Lv,Jing Zhang,Qianqian Ma,Qiuhua Li,Li Song,Li Gong,Yun-Liang Zhu,Xiang-Yuan Li,Yanlei Hao,Yan Yang +10 more
TL;DR: Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these differentially expressed circRNAs were strongly associated with inflammation, metabolism, and immune responses, which are all risk factors for Alzheimer’s disease.
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Circular RNA circLMO1 Suppresses Cervical Cancer Growth and Metastasis by Triggering miR-4291/ACSL4-Mediated Ferroptosis
Rongying Ou,Shun Lu,Luhui Wang,Yebo Wang,Mingfen Lv,Tiane Li,Yunsheng Xu,Jieqiang Lu,Ren-Shan Ge +8 more
TL;DR: CircLMO1 acted as a tumor suppressor of cervical cancer by regulating miR-4291/ACSL4-mediated ferroptosis, and could be a promising biomarker for the clinical management of cervicalcancer.
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Circular RNA Foxo3 Relieves Myocardial Ischemia/Reperfusion Injury by Suppressing Autophagy via Inhibiting HMGB1 by Repressing KAT7 in Myocardial Infarction
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Circular RNAs: The crucial regulatory molecules in colorectal cancer.
TL;DR: This review systematically introduces the CRC-related circRNAs and their functional mechanisms, as well as the potential applications for CRC diagnosis and prognosis.
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Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
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miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
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TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
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Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.