Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Book ChapterDOI
Circular RNAs in Cancer
Susanne Lux,Lars Bullinger +1 more
TL;DR: This chapter will review cancer-associated and cancer-specific circRNAs, some of which have oncogenic or tumor-suppressive potential, and the opportunity to use circ RNAs as biomarkers and potential therapeutic targets in cancer is discussed.
Journal ArticleDOI
Tissue-Dependent Expression and Translation of Circular RNAs with Recombinant AAV Vectors In Vivo.
Rita M. Meganck,Erin K. Borchardt,Ruth M. Castellanos Rivera,Miranda L. Scalabrino,Jeremy E. Wilusz,William F. Marzluff,Aravind Asokan +6 more
TL;DR: The design and characterization of recombinant adeno-associated viral (AAV) vectors packaging transgene cassettes containing intronic sequences that promote backsplicing to generate circularized RNA transcripts are described and demonstrated.
Journal ArticleDOI
Microarray Expression Profile of Circular RNAs in Heart Tissue of Mice with Myocardial Infarction-Induced Heart Failure
TL;DR: The distinct expression patterns of circulatory circ RNAs during HF indicate that circRNAs may actively respond to stress and thus serve as biomarkers of HF diagnosis and treatment.
Journal ArticleDOI
Roles of Circular RNAs in Neurologic Disease
Yiye Shao,Yinghui Chen +1 more
TL;DR: Circular RNAs are a novel type of endogenous noncoding RNA receiving increasing attention that act as a natural microRNA sponges that repress the activity of corresponding miRNAs by binding with them, thus regulating target genes.
Journal ArticleDOI
CircPrimer: a software for annotating circRNAs and determining the specificity of circRNA primers.
TL;DR: circPrimer is a user-friendly tool for exploring circRNAs that does not require special user skills and enables users to extract the spliced sequences and genomic sequences of any circRNA, including novel circ RNAs.
References
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A coding-independent function of gene and pseudogene mRNAs regulates tumour biology
Laura Poliseno,Leonardo Salmena,Jiangwen Zhang,Brett S. Carver,William J. Haveman,Pier Paolo Pandolfi +5 more
TL;DR: It is found that PTENP1 is biologically active as it can regulate cellular levels of PTEN and exert a growth-suppressive role, and this analysis extended to other cancer-related genes that possess pseudogenes, and revealed a non-coding function for mRNAs.
Journal ArticleDOI
Circular transcripts of the testis-determining gene Sry in adult mouse testis
Blanche Capel,Amanda Swain,Silvia K. Nicolis,Adam Hacker,Michael A. Walter,Peter Koopman,Peter N. Goodfellow,Robin Lovell-Badge +7 more
TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
Journal ArticleDOI
Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
Thomas B. Hansen,Erik D Wiklund,Erik D Wiklund,Jesper B. Bramsen,Sune B. Villadsen,Aaron L. Statham,Susan J. Clark,Jørgen Kjems +7 more
TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
Journal ArticleDOI
Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.