Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Circular RNAs: A novel type of biomarker and genetic tools in cancer
TL;DR: The origins, characteristics and functions of circRNA and how they work as miRNA sponges, gene transcription and expression regulators, RBP sponge in cancer as well as current research methods of circRNAs are discussed, providing evidence for the significance ofcircRNAs in cancer diagnosis and clinical treatment.
Journal ArticleDOI
Comprehensive circular RNA profiles in plasma reveals that circular RNAs can be used as novel biomarkers for systemic lupus erythematosus.
Haixia Li,Kaifei Li,Weinan Lai,Xin Li,Hongxia Wang,Jia Yang,Shuai Chu,Haifang Wang,Chun-Min Kang,Yu-Rong Qiu +9 more
TL;DR: The comprehensive expression profiles of circRNAs in SLE patients plasma are illustrated and the foundations to developcircRNAs as novel non-invasive biomarkers for SLE disease in the future are laid.
Journal ArticleDOI
Advances in circular RNAs and their roles in breast Cancer
Xuehui Wang,Lin Fang +1 more
TL;DR: Emerging evidence suggests that the biological functions of circRNAs including serving as ceRNAs or miRNA sponges, interacting with proteins, regulating gene transcription and translation, suggesting that circ RNAs will be novel biomarkers and targets for the diagnosis and prognosis of diseases.
Journal ArticleDOI
The potential of using blood circular RNA as liquid biopsy biomarker for human diseases
Guoxia Wen,Tong Zhou,Wanjun Gu +2 more
TL;DR: This review focuses on the translational potential of using human blood circRNAs as liquid biopsy biomarkers for human diseases, and highlights their abundant expression, essential biological functions and significant correlations to human diseases in various components of peripheral blood.
Journal ArticleDOI
The emerging role and clinical implication of human exonic circular RNA.
Dongbin Lyu,Shenglin Huang +1 more
TL;DR: Current knowledge of human circRNAs is summarized and the emerging role and clinical implication of these multifarious transcripts are discussed.
References
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Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
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TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
Journal ArticleDOI
Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.