Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Identification of functional circRNA/miRNA/mRNA regulatory network for exploring prospective therapy strategy of colorectal cancer
Dongping Xu,Yutong Wu,Xiufang Wang,Xiaoyun Hu,Wenyan Qin,Yalun Li,Yilin Wang,Zhen Zhang,Senxu Lu,Tong Sun,Zhikun Wu,Dandan Fu,Boshi Fu,Jing Zhang,Qiuchen Chen,Minjie Wei,Lin Zhao,Huizhe Wu +17 more
TL;DR: This study discovered the differential expressed circRNAs between CRC tissues and matched adjacent using deep RNA sequencing and confirmed the DECs expression by combining with another Gene Expression Omnibus dataset.
Journal ArticleDOI
Circular RNA in colorectal cancer.
Anthony Li,Anthony Li,Wei Cen Wang,Vivian C. McAlister,Vivian C. McAlister,Qinfeng Zhou,Qinfeng Zhou,Xiufen Zheng +7 more
TL;DR: In this article, a review summarizes the role of specific circRNAs in colorectal cancer and evaluates their potential value as therapeutic targets and biomarkers for CRC, aiming to provide insight in the development of therapy and clinical decision-making.
Journal ArticleDOI
Differential Expression of CircRNAs in Embryonic Heart Tissue Associated with Ventricular Septal Defect.
Heng Liu,Yin Hu,Bin Zhuang,Jing Yin,Xiao Hui Chen,Juan Wang,Mengmeng Li,Jing Xu,Xing Yun Wang,Zhang Bin Yu,Shu Ping Han +10 more
TL;DR: Combined with the microarray results and previous researches, circRNAs may contribute to the occurrence of VSD by acting as miRNA sponges or by binding proteins, these possible roles forcircRNAs in VSD require elucidation in additional studies.
Journal ArticleDOI
Erratum to: Statistically based splicing detection reveals neural enrichment and tissue-specific induction of circular RNA during human fetal development [Genome Biology, 16, (2016) (126)], DOI: 10.1186/s13059-015-0690-5
Linda Szabo,Robert Morey,Nathan J. Palpant,Peter L. Wang,Nastaran Afari,Chuan Jiang,Mana M. Parast,Charles E. Murry,Louise C. Laurent,Julia Salzman +9 more
TL;DR: The authors have now analysed appropriate positive and negative controls using their method, KNIFE, and find_circ, and provided a list of accession codes for the ENCODE data they analysed.
Journal ArticleDOI
Screening for differentially expressed circRNA between Kashin-Beck disease and osteoarthritis patients based on circRNA chips.
Ying Wang,Cuiyan Wu,Yanan Zhang,Yimin Yang,Zhiwei Ren,Mikko J. Lammi,Mikko J. Lammi,Xiong Guo +7 more
TL;DR: The ROC curve demonstrated that hsa_circ_0020014_CBC1 in peripheral blood could distinguish patients with KBD and OA, and is a potential biomarker for differential diagnosis between these two diseases.
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