Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Long Non-coding RNAs in the Cytoplasm.
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Circular RNA-ITCH Suppresses Lung Cancer Proliferation via Inhibiting the Wnt/β-Catenin Pathway
TL;DR: The results suggested that cir-ITCH may play an inhibitory role in lung cancer progression by enhancing its parental gene, ITCH, expression.
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The Output of Protein-Coding Genes Shifts to Circular RNAs When the Pre-mRNA Processing Machinery Is Limiting.
Dongming Liang,Deirdre C. Tatomer,Zheng Luo,Huang Wu,Li Yang,Ling-Ling Chen,Sara Cherry,Jeremy E. Wilusz +7 more
TL;DR: Results demonstrate that inhibition or slowing of canonical pre-mRNA processing events shifts the steady-state output of protein-coding genes toward circular RNAs, because nascent RNAs become directed into alternative pathways that lead to circular RNA production.
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CSCD: a database for cancer-specific circular RNAs
Siyu Xia,Jing Feng,Ke Chen,Yanbing Ma,Jing Gong,Fangfang Cai,Yuxuan Jin,Yang Gao,Linjian Xia,Hong Chang,Lei Wei,Leng Han,Chunjiang He +12 more
TL;DR: A cancer-specific circRNA database is constructed (CSCD) that could significantly contribute to the research for the function and regulation of cancer-associated circRNAs.
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Non-Coding RNAs and their Integrated Networks.
TL;DR: This review discusses the distinct types of ncRNAs, including housekeeping n cRNAs and regulatory nc RNAs, their versatile functions and interactions, transcription, translation, and modification, and summarizes the integrated networks of n cRNA interactions, providing a comprehensive landscape of nCRNAs regulatory roles.
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TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
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Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.