Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Hsa_circ_0002577 promotes endometrial carcinoma progression via regulating miR-197/CTNND1 axis and activating Wnt/β-catenin pathway
TL;DR: It is shown that hsa_circ_0002577 expression was significantly upregulated in EC tissues, and high hsa-circ-00025 77 expression was associated with advanced FIGO stage, lymph node metastasis, and poor overall survival rate of EC patients, and the oncogenic effects of hSA_circ-2577 were attributed to the regulation of miR-197/CTNND1/Wnt/β-catenin axis.
Journal ArticleDOI
Circular RNA circHIAT1 inhibits cell growth in hepatocellular carcinoma by regulating miR-3171/PTEN axis.
TL;DR: A positive correlation between the downregulation of circHIAT1 and the poor overall survival of HCC patients is determined and rescue assays demonstrated the role of circ HIAT1/miR-3171/PTEN pathway in regulating HCC cell growth.
Journal ArticleDOI
The RNA world of human ageing.
Juan Carlos Gomez-Verjan,Edgar Ricardo Vázquez-Martínez,Nadia Alejandra Rivero-Segura,R H Medina-Campos +3 more
TL;DR: Overall, human transcriptomics research indicates that although there are common alterations of the regular expression patterns of the energetic and oxidative metabolism, extracellular matrix regulation and inflammation pathways, ageing seems to be gender and tissue-specific in general.
Journal ArticleDOI
ciRS-7 targeting miR-135a-5p promotes neuropathic pain in CCI rats via inflammation and autophagy.
Wei Cai,Yang Zhang,Zhen Su +2 more
TL;DR: Results indicate that ciRS-7 associates with the progress of neuropathic pain partly by upregulated the expression level of autophagy and inflammation in the CCI rats, and it is found ci RS-7 regulates neuropathicPain progress by sponging to miR-135a-5p.
Journal ArticleDOI
CircRNA expression profiles and function prediction in peripheral blood mononuclear cells of patients with acute ischemic stroke
TL;DR: Patients with AIS differentially express certain circRNAs in PBMCs, which may be diagnostic biomarkers or potential therapeutic targets, and Kyoto Encyclopedia of Genes and Genomes pathway enrichment and gene ontology analyses indicated that the aberrantly expressed circ RNAs participated in many pathophysiological processes of AIS.
References
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TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
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Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
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