Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Journal ArticleDOI
Microarray is an efficient tool for circRNA profiling.
Shasha Li,Shuaishuai Teng,Junquan Xu,Guannan Su,Yu Zhang,Jianqing Zhao,Suwei Zhang,Haiyan Wang,Wenyan Qin,Zhi John Lu,Guo Yong,Qianyong Zhu,Dong Wang +12 more
TL;DR: CircRNAs could be efficiently profiled by circRNA microarray to target their reported back-splice sites in interested samples and be validated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) or RNA-seq.
Journal ArticleDOI
CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs.
Tim Schneider,Lee-Hsueh Hung,Silke Schreiner,Stefan Starke,Heinrich Eckhof,Oliver Rossbach,Stefan Reich,Jan Medenbach,Albrecht Bindereif +8 more
TL;DR: The data suggest that specific circRNP families exist defined by a common protein component, and provides a general approach to identify circRNPs with a given protein component.
Journal ArticleDOI
Circular RNAs: Characterization, cellular roles, and applications
Chu-Xiao Liu,Ling-Ling Chen +1 more
TL;DR: Liu et al. as mentioned in this paper reviewed the growing toolbox to characterize circular RNA, the resulting emerging understanding of their biological roles, and how circular RNA are being utilized for biomedical applications.
Journal ArticleDOI
Tracing the expression of circular RNAs in human pre-implantation embryos
Yujiao Dang,Liying Yan,Liying Yan,Boqiang Hu,Xiaoying Fan,Yixin Ren,Yixin Ren,Rong Li,Rong Li,Ying Lian,Ying Lian,Jie Yan,Jie Yan,Qingqing Li,Yan Zhang,Yan Zhang,Min Li,Min Li,Xiulian Ren,Xiulian Ren,Jin Huang,Jin Huang,Yuqi Wu,Yuqi Wu,Ping Liu,Ping Liu,Lu Wen,Chen Zhang,Yanyi Huang,Fuchou Tang,Jie Qiao +30 more
TL;DR: This study reports the first analysis of the whole transcriptome comprising both polyA+ mRNAs and polyA– RNAs including circ RNAs during human pre-implantation development, providing an invaluable resource for analyzing the unique function and complex regulatory mechanisms of circRNAs during this process.
Patent
Modified polynucleotides for the production of cosmetic proteins and peptides
TL;DR: In this article, compositions, methods, processes, kits and devices for the design, preparation, manufacture and/or formulation of modified mRNA (mmRNA) molecules encoding at least one cosmetic polypeptide of interest.
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