Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Long Noncoding RNAs with snoRNA Ends
Qing-Fei Yin,Li Yang,Yang Zhang,Jian-Feng Xiang,Yue-Wei Wu,Gordon G. Carmichael,Ling-Ling Chen +6 more
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Aberrant RNA splicing in cancer; expression changes and driver mutations of splicing factor genes
Anita Sveen,Anita Sveen,Sami Kilpinen,A Ruusulehto,Ragnhild A. Lothe,Ragnhild A. Lothe,Rolf Inge Skotheim,Rolf Inge Skotheim +7 more
TL;DR: This review provides an overview of aberrant RNA splicing and its regulation in cancer, and proposes 24 novel cancer-critical splicing factors predicted from somatic mutations.
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The novel roles of circRNAs in human cancer
TL;DR: The characteristics and types of circRNAs are summarized, the biogenesis ofcircRNAs is introduced, the emerging functions and databases on circ RNAs are discussed, and the current challenges of CircRNAs studies are presented.
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LNCcation: lncRNA localization and function.
TL;DR: In this paper, the authors summarize current knowledge of long noncoding RNAs subcellular localization, factors controlling their localization, emerging themes, including the role of lncRNA isoforms and the involvement of LncRNAs in phase separation bodies, and the implications of LNCRNA localization on their function and on cellular behavior.
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Circular RNAs in cancer: novel insights into origins, properties, functions and implications.
Jingqiu Li,Jie Yang,Ping Zhou,Yanping Le,Chengwei Zhou,Shaomin Wang,Dazhi Xu,Hui Kuan Lin,Zhaohui Gong +8 more
TL;DR: The current understanding of the roles of circRNAs in cancers and the potential implications of circRNA-miRNA axes are reviewed, with a focus on cancer targeted therapy.
References
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TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
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TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
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Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
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