Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Journal ArticleDOI
Emerging function and potential diagnostic value of circular RNAs in cancer.
Xianglun Cui,Jianxun Wang,Zongjun Guo,Mengyang Li,Mingyu Li,Si Liu,Haoran Liu,Wenjing Li,Xunhua Yin,Jiaping Tao,Wenhua Xu +10 more
TL;DR: The latest research on circRNAs is summarized, including its classification and biogenesis, mechanism and functions, as well as circ RNAs in different cancers, as a potential biomarker for cancer diagnosis.
Journal ArticleDOI
Circ2Disease: a manually curated database of experimentally validated circRNAs in human disease.
TL;DR: With the rapidly increasing interest in circRNAs, Circ2Disease will significantly improve the understanding of circRNA deregulation in diseases and is a useful resource for studying posttranscriptional regulatory roles ofcircRNAs in human diseases.
Journal ArticleDOI
Identification of Circular RNAs and Their Targets in Leaves of Triticum aestivum L. under Dehydration Stress.
TL;DR: A possible connection between the regulations of circRNAs with the expressions of functional genes in wheat leaves associated with dehydration resistance is revealed.
Journal ArticleDOI
Circular RNAs and gastrointestinal cancers: Epigenetic regulators with a prognostic and therapeutic role.
Parisa Naeli,Mohammad Hossein Pourhanifeh,Mohammad Reza Karimzadeh,Zahra Shabaninejad,Zahra Shabaninejad,Ahmad Movahedpour,Hossein Tarrahimofrad,Hamid Reza Mirzaei,Hassan Hassani Bafrani,Amir Savardashtaki,Hamed Mirzaei,Michael R. Hamblin +11 more
TL;DR: The properties and biogenesis of circRNAs are described, recent reports about circRNA functions, expression status, and their potential to be used as biomarkers in GI cancers including, gastric cancer, colorectal cancer, esophageal cancers, hepatocellular carcinoma, gallbladder cancer and pancreatic cancer are summarized.
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CircRNA in cancer: Fundamental mechanism and clinical potential.
Liang Chen,Ge Shan +1 more
TL;DR: A review of the characteristics, types, biogenesis, and functions of circRNAs can be found in this article, where the authors outline a series of the most recently studied CircRNAs and their functional mechanisms in multiple cancer types with future perspectives.
References
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TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
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Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
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TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
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Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.