Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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Journal ArticleDOI
Regulation of microRNAs function by circular RNAs in human cancer.
TL;DR: The molecular characteristics and biogenesis of circRNAs are reviewed, with a focus on newly identified circ RNAs that may play an important role in human cancer, through their regulation of miR expression.
Journal ArticleDOI
Computational approaches for circular RNA analysis.
TL;DR: An overview on different computational analysis tools and pipelines that became available throughout the last years of circRNAs research is provided and a set of valuable validation strategies, in silico as well as in vitro‐based approaches are provided.
Journal ArticleDOI
PlantCircNet: a database for plant circRNA–miRNA–mRNA regulatory networks
TL;DR: The identification of several circRNAs to play potential regulatory roles in flower development and response to environmental stress from regulatory networks related with miR156a and AT5G59720, respectively were identified.
Journal ArticleDOI
Long non-coding RNAs in vascular biology and disease.
TL;DR: This review summarizes the current knowledge of lncRNA and circRNA functions in vascular biology and disease and discusses their potential use as biomarkers.
Journal ArticleDOI
Circular RNA profiling provides insights into their subcellular distribution and molecular characteristics in HepG2 cells.
Ju Zhang,Xiuli Zhang,Xiuli Zhang,Cuidan Li,Cuidan Li,Liya Yue,Nan Ding,Tim Riordan,Li Yang,Li Yang,Yang Li,Charles Jen,Sen Lin,Dongsheng Zhou,Fei Chen,Fei Chen +15 more
TL;DR: This study comprehensively analyzed the subcellular distribution/characteristics and the transport mechanism of circRNA, and found that the exosome could selectively package the circRNAs containing the purine-rich 5ʹ-GMWGVWGRAG-3ʹ motif, with the characteristic of ‘garbage dumping’ and ‘intercellular signaling’ functions.
References
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Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
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TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
Journal ArticleDOI
Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.