Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
TLDR
By deep sequencing of RNA from a variety of normal and malignant human cells, this work suggests that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.Abstract:
Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.read more
Citations
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CircRNA expression pattern and circRNA-miRNA-mRNA network in the pathogenesis of nonalcoholic steatohepatitis
TL;DR: Investigation of the circRNA expression pattern in NASH and construction of circRNA-miRNA-mRNA network for in-depth mechanism exploration suggest circRNA profile may serve as candidate for NASH diagnosis and circRNA -miRNA -mRNA pathway may provide novel mechanism for NASh.
Journal ArticleDOI
ANRIL: A lncRNA at the CDKN2A/B Locus With Roles in Cancer and Metabolic Disease.
TL;DR: Evidence suggests that ANRIL gain of function increases atherosclerosis; in diabetes, a risk-SNP reduced the pancreatic beta cell proliferation index; and diverse cell-type-dependent results suggest it is necessary to perform studies in the relevant primary human tissue for each disease.
Journal ArticleDOI
Androgen-responsive circular RNA circSMARCA5 is up-regulated and promotes cell proliferation in prostate cancer.
Zhe Kong,Xuechao Wan,Yalong Zhang,Pu Zhang,Yingyi Zhang,Xiaona Zhang,Xiaoxiang Qi,Hai Wu,Jianfeng Huang,Yao Li +9 more
TL;DR: Functional experiments showed circ-SMARCA5 acted as an oncogene in prostate cancer by promoting cell cycle and inhibiting cell apoptosis and this study provided useful information for exploring circRNAs as potential therapeutic and prognostic targets for prostate cancer.
Patent
Modified polynucleotides for the production of oncology-related proteins and peptides
Stephane Bancel,Tirtha Chakraborty,Fougerolles Antonin De,Sayda M. Elbashir,Matthias John,Atanu Roy,Susan Whoriskey,Kristy M. Wood,Paul Hatala,Jason P. Schrum,Kenechi Ejebe,Jeff Lynn Ellsworth,Justin Guild +12 more
TL;DR: In this paper, the authors describe compositions including polynucleotides encoding polypeptides which have been chemically modified by replacing the uridines with 1-methyl-pseudouridine to improve one or more of the stability and/or clearance in tissues.
Journal ArticleDOI
Roles of circRNAs in the tumour microenvironment
Qiuge Zhang,Weiwei Wang,Quanbo Zhou,Chen Chen,Weitang Yuan,Jinbo Liu,Xiaoli Li,Zhenqiang Sun +7 more
TL;DR: The prospect of targeting circRNAs as an essential strategy to regulate TME, overcome cancer resistance and improve therapeutic outcomes is emphasized.
References
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Journal ArticleDOI
A coding-independent function of gene and pseudogene mRNAs regulates tumour biology
Laura Poliseno,Leonardo Salmena,Jiangwen Zhang,Brett S. Carver,William J. Haveman,Pier Paolo Pandolfi +5 more
TL;DR: It is found that PTENP1 is biologically active as it can regulate cellular levels of PTEN and exert a growth-suppressive role, and this analysis extended to other cancer-related genes that possess pseudogenes, and revealed a non-coding function for mRNAs.
Journal ArticleDOI
Circular transcripts of the testis-determining gene Sry in adult mouse testis
Blanche Capel,Amanda Swain,Silvia K. Nicolis,Adam Hacker,Michael A. Walter,Peter Koopman,Peter N. Goodfellow,Robin Lovell-Badge +7 more
TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
Journal ArticleDOI
Mis-splicing yields circular RNA molecules.
TL;DR: To the knowledge, this is the first case of circular transcripts being processed from nuclear pre‐mRNA in eukaryotes, and might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.
Journal ArticleDOI
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
Thomas B. Hansen,Erik D Wiklund,Erik D Wiklund,Jesper B. Bramsen,Sune B. Villadsen,Aaron L. Statham,Susan J. Clark,Jørgen Kjems +7 more
TL;DR: This study provides the first evidence for non‐coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.
Journal ArticleDOI
Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
TL;DR: The results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRil expression and/or structure.