Eivind Valen

TL;DR: It is shown that enhancers share properties with CpG-poor messenger RNA promoters but produce bidirectional, exosome-sensitive, relatively short unspliced RNAs, the generation of which is strongly related to enhancer activity.

TL;DR: For example, the authors mapped transcription start sites (TSSs) and their usage in human and mouse primary cells, cell lines and tissues to produce a comprehensive overview of mammalian gene expression across the human body.

TL;DR: An online tool, CHOPCHOP, that uses efficient sequence alignment algorithms to minimize search times, and rigorously predicts off-target binding of single-guide RNAs (sgRNAs) and TALENs, making it a valuable tool for genome engineering.

TL;DR: This major update of CHOPCHOP introduces functionality for targeting RNA with Cas13, which includes support for alternative transcript isoforms and RNA accessibility predictions, and incorporates new DNA targeting modes, including CRISPR activation/repression, targeted enrichment of loci for long-read sequencing, and prediction of Cas9 repair outcomes.

TL;DR: Improved methods and detailed protocols make Cas9-mediated mutagenesis an attractive approach for labs of all sizes and increase rates of mutagenisation by implementing several novel approaches.