F
Feng Zhang
Researcher at Fudan University
Publications - 2715
Citations - 225233
Feng Zhang is an academic researcher from Fudan University. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 172, co-authored 1278 publications receiving 181865 citations. Previous affiliations of Feng Zhang include Cincinnati Children's Hospital Medical Center & Nanjing Medical University.
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Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems
Sergey Shmakov,Omar O. Abudayyeh,Kira S. Makarova,Yuri I. Wolf,Jonathan S. Gootenberg,Ekaterina Semenova,Leonid Minakhin,Julia Joung,Silvana Konermann,Konstantin Severinov,Konstantin Severinov,Konstantin Severinov,Feng Zhang,Eugene V. Koonin +13 more
TL;DR: Comparison analysis indicates that Class 2 CRISPR-Cas systems evolved on multiple occasions through recombination of Class 1 adaptation modules with effector proteins acquired from distinct mobile elements.
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Sequence determinants of improved CRISPR sgRNA design
Han Xu,Tengfei Xiao,Chen-Hao Chen,Wei Li,Clifford A. Meyer,Qiu Wu,Qiu Wu,Di Wu,Le Cong,Le Cong,Feng Zhang,Feng Zhang,Jun Liu,Myles Brown,Myles Brown,X. Shirley Liu +15 more
TL;DR: This work derived a new sequence model for predicting sgRNA efficiency in CRISPR/Cas9 knockout experiments and suggested new features including a preference for cytosine at the cleavage site that facilitate the genome-wide design of improved sg RNA for both knockout and CRISpri/a studies.
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Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri
Feng Zhang,Matthias Prigge,Florent Beyrière,Satoshi P. Tsunoda,Joanna Mattis,Ofer Yizhar,Peter Hegemann,Karl Deisseroth +7 more
TL;DR: A cation-conducting channelrhodopsin (VChR1) from Volvox carteri that can drive spiking at 589 nm, with excitation maximum red-shifted ∼70 nm compared with ChR2 is described, thereby defining a functionally distinct third category of microbial rhodopin proteins.
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Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)
Jeffry D. Sander,Elizabeth J. Dahlborg,Mathew J. Goodwin,Lindsay Cade,Feng Zhang,Daniel Cifuentes,Shaun J. Curtin,Jessica S. Blackburn,Stacey Thibodeau-Beganny,Yiping Qi,Christopher J. Pierick,Ellen J. Hoffman,Morgan L. Maeder,Cyd Khayter,Deepak Reyon,Drena Dobbs,David M. Langenau,Robert M. Stupar,Antonio J. Giraldez,Daniel F. Voytas,Randall T. Peterson,Randall T. Peterson,Jing-Ruey J. Yeh,J. Keith Joung +23 more
TL;DR: Context-dependent assembly (CoDA) is described, a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis and rapidly altered 20 genes in Danio rerio, Arabidopsis thaliana and Glycine max.
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A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models
Qiurong Ding,Youn-Kyoung Lee,Esperance A. Schaefer,Derek T. Peters,Adrian Veres,Kevin J. Kim,Nicolas Kuperwasser,Nicolas Kuperwasser,Daniel L. Motola,Torsten B. Meissner,William T. Hendriks,Marta Trevisan,Rajat M. Gupta,Rajat M. Gupta,Annie Moisan,Eric Banks,Max Friesen,Robert T. Schinzel,Fang Xia,Alexander Tang,Yulei Xia,Emmanuel Figueroa,Amy Wann,Tim Ahfeldt,Laurence Daheron,Feng Zhang,Feng Zhang,Lee L. Rubin,Lee F. Peng,Raymond T. Chung,Kiran Musunuru,Chad A. Cowan +31 more
TL;DR: Transcription activator-like effector nucleases (TALENs) are a new class of engineered nucleases that are easier to design to cleave at desired sites in a genome than previous types of nucleases as discussed by the authors.