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Laser speckle contrast imaging in biomedical optics

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TLDR
The underlying physics of speckle contrast imaging is reviewed, recent developments to improve the quantitative accuracy of blood flow measures are discussed and applications in neuroscience, dermatology and ophthalmology are reviewed.
Abstract
First introduced in the 1980s, laser speckle contrast imaging is a powerful tool for full-field imaging of blood flow. Recently laser speckle contrast imaging has gained increased attention, in part due to its rapid adoption for blood flow studies in the brain. We review the underlying physics of speckle contrast imaging and discuss recent developments to improve the quantitative accuracy of blood flow measures. We also review applications of laser speckle contrast imaging in neuroscience, dermatology and ophthalmology.

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References
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Temporal statistical analysis of laser speckle images and its application to retinal blood-flow imaging

TL;DR: The results from flow phantoms and in vivo rat retinas demonstrated that the TS-LSI signals are temporally statistically independent and TS- LSI minimizes stationary-speckle contamination.
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Optical imaging of the spatiotemporal dynamics of cerebral blood flow and oxidative metabolism in the rat barrel cortex

TL;DR: The results provide evidence that the increase in cerebral oxidative metabolism in response to sensory stimulation is considerably faster and more localized than the CBF response, and suggests that future developments in functional imaging concentrating on the metabolic response promise an increased spatial resolution.
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Linear response range characterization and in vivo application of laser speckle imaging of blood flow dynamics.

TL;DR: It is implied that LSI can provide accurate wide-field maps of microvascular blood flow rate dynamics and highlight heterogeneities in flow response to the application of exogenous agents.
Journal ArticleDOI

Quantitative modeling of laser speckle imaging

TL;DR: In this paper, the authors analyzed the image formation and dynamic properties in laser speckle imaging (LSI) both experimentally and with Monte-Carlo simulation and showed that the spatial resolution and the signal itself are both significantly affected by scattering from the turbid environment.
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