Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
High-speed single-molecule tracking of CXCL13 in the B-Follicle
Helen Miller,Helen Miller,Jason Cosgrove,Adam J. M. Wollman,Emily Taylor,Zhaokun Zhou,Peter O'Toole,Mark Coles,Mark Coles,Mark C. Leake +9 more
TL;DR: A high-speed single-molecule tracking approach that outperforms competing tools for determining molecular mobility of fluorescence correlation spectroscopy and fluorescence recovery after photobleaching for evaluation of diffusion is demonstrated and obtained an increased understanding of CXCL13 bioavailability within the follicle.
Journal ArticleDOI
3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum.
Jaroslaw Sedzicki,Therese Tschon,Shyan Huey Low,Kevin Willemart,Kenneth N. Goldie,Jean-Jacques Letesson,Henning Stahlberg,Christoph Dehio +7 more
TL;DR: 3D correlative light and electron microscopy (3D-CLEM) reveals that replicative Brucella-containing vacuoles (rBCVs) are continuous with the ER, indicating that the pathogen replicates inside bona fide host ER.
Patent
Embedded pupil function recovery for fourier ptychographic imaging devices
TL;DR: In this article, the Fourier ptychographic imaging system, devices, and methods that implement an embedded pupil function recovery are described and compared to the ones described in this paper.
Journal ArticleDOI
Advances in high-resolution microscopy for the study of intracellular interactions with biomaterials
TL;DR: This review evaluates the advantages and disadvantages of different forms of electron microscopy and super-resolution microscopy in elucidating how biomaterial surface chemistry and topography can affect intracellular events at the nanoscale.
Patent
System and method providing intracoronary laser speckle imaging for the detection of vulnerable plaque
TL;DR: In this article, a wave-guiding arrangement was proposed to reduce the crosstalk between two wave-guide arrangements associated with one another that are configured to receive a further electro-magnetic radiation reflected from the tissue and transmit at least one speckle pattern associated with the further EM radiation.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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