Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Patent
Systems and methods for 3-dimensional interferometric microscopy
TL;DR: In this paper, an optical system is configured to produce images of an optical source in a first dimension and a second dimension substantially orthogonal to the first dimension at each detector at a given time.
Journal ArticleDOI
STED-TEM Correlative Microscopy Leveraging Nanodiamonds as Intracellular Dual-Contrast Markers.
Neeraj Prabhakar,Neeraj Prabhakar,Markus Peurla,Sami Koho,Sami Koho,Takahiro Deguchi,Tuomas Näreoja,Huan-Cheng Chang,Jessica M. Rosenholm,Pekka Hänninen +9 more
TL;DR: It is demonstrated how the FNDs can be used as dual-contrast labels-and together with automatic image registration tool SuperTomo, for precise image correlation-in high-resolution stimulated emission depletion (STED)/confocal and transmission electron microscopy (TEM) correlative microscopy experiments.
Journal ArticleDOI
Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells.
TL;DR: A two-step kinetic model for sterol transport between PM and recycling endosomes and the suitability of BChol for determining transport of lipoprotein-derived sterol using electron microscopy (EM) is highlighted and shown that this approach ideally complements fluorescence studies.
Journal ArticleDOI
Bidirectional intraflagellar transport is restricted to two sets of microtubule doublets in the trypanosome flagellum
Eloïse Bertiaux,Eloïse Bertiaux,Adeline Mallet,Adeline Mallet,Cécile Fort,Cécile Fort,Thierry Blisnick,Serge Bonnefoy,Jamin Jung,Moara Lemos,Sergio Marco,Sergio Marco,Sue Vaughan,Sylvain Trépout,Sylvain Trépout,Jean-Yves Tinevez,Philippe Bastin +16 more
TL;DR: High-resolution live imaging of cells expressing mNeonGreen::IFT81 or GFP::IFT52 revealed for the first time IFT trafficking on two parallel lines within the flagellum of the protist Trypanosoma brucei.
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Nanoscale mechanobiology of cell adhesions
Shumin Xia,Pakorn Kanchanawong +1 more
TL;DR: Current insights and key open questions regarding the nanoscale structure and function relationship of cell adhesions are discussed, focusing on recent progresses in characterizing their composition, spatial organization, and cytomechanical operation.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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