Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Optical imaging of individual biomolecules in densely packed clusters
TL;DR: DNA-PAINT, a super-resolution fluorescence microscopy technique that exploits programmable transient oligonucleotide hybridization, can be used to image densely packed triangular lattice patterns with molecular-level resolution and ångström-level precision.
Posted Content
Imaging through glass diffusers using densely connected convolutional networks
TL;DR: In this article, a convolutional neural network architecture called IDiffNet is proposed for the problem of imaging through diffuse media and demonstrate that IDiffnet has superior generalization capability through extensive tests with well-calibrated diffusers.
Journal ArticleDOI
Superresolution by image scanning microscopy using pixel reassignment
TL;DR: The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied and it is shown how the method can be employed if there is a Stokes shift in fluorescence emission wavelength.
Journal ArticleDOI
Light-sheet microscopy: a tutorial
TL;DR: This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective, and emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms.
Patent
Process and apparatus for a wavelength tuning source
TL;DR: An apparatus and source arrangement for filtering an electromagnetic radiation can be provided which may include at least one spectral separating arrangement configured to physically separate one or more components of the electromagnetic radiation based on a frequency of the EM radiation as discussed by the authors.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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